PDOX Bibliography by tumor type

A selection of published PDOX studies. These studies are available by request.

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PDOX – Patient Derived Orthotopic Xenografts™

General

  1. Dutton, G. AntiCancer Inc. scientists identify a key governing step in the metastasis of cancer. Genetic Engineering News 16, 1, January 15, 1996.
  2. Holzman, D. Of mice and metastasis: A new for-profit model emerges. Journal of the National Cancer Institute 88, 396-397, 1996.
  3. Riordan, T. A technique is said to ease attachment of tumors to mice, making them ‘little cancer patients.’ New York Times, “Patents” Column, March 4, 1996.
  4. Hoffman, R.M. Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Investigational New Drugs 17, 343-359, 1999.
  5. Dove, A. Illuminating cancer models. Nature Biotechnology 18, 261, 2000.
  6. McCann, J. Jellyfish protein gives new glow to tumor imaging. Journal of the National Cancer Institute 93, 976-977, 2001.
  7. Garber, K. Realistic rodents? Debate grows over new mouse models of cancer. Journal of the National Cancer Institute 98, 1176-1178, 2006.
  8. Garber, K. From human to mouse and back: “Tumorgraft” models surge in popularity. Journal of the National Cancer Institute 101, 6-8, 2009.
  9. Hoffman, R.M. Patient-derived orthotopic xenografts: better mimic of metastasis than subcutaneous xenografts. Nature Reviews Cancer 15, 451-452, 2015.

Bladder

  1. Fu, X., Theodorescu, D., Kerbel, R.S., and Hoffman, R.M. Extensive multi-organ metastasis following orthotopic onplantation of histologically-intact human bladder carcinoma tissue in nude mice. Int. J. Cancer 49, 938-939, 1991.
  2. Fu, X., and Hoffman, R.M. Human RT-4 bladder carcinoma is highly metastatic in nude mice and comparable to ras-H-transformed RT-4 when orthotopically onplanted as histologically intact tissue. Int. J. Cancer 51, 989-991, 1992.
  3. Chang, S-G., Kim, J.I., Jung, J-C., Rho, Y-S., Lee, K-T., An, Z., Wang, X., and Hoffman, R.M. Antimetastatic activity of the new platinum analog {Pt(cis-dach)(DPPE)×2NO3} in a metastatic model of human bladder cancer. Anticancer Res. 17, 3239-3242, 1997.
  4. Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman, R.M., Benedict, W.F. Visualizing superficial human bladder cancer cell growth in vivo by green fluorescent protein expression. Cancer Gene Therapy 9, 681-686, 2002.

Bone Cancer & Metastasis

  1. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression. Cancer Research 58, 4217-4221, 1998.
  2. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Research 59, 781-786, 1999
  3. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Research 5, 3549-3559, 1999
  4. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000
  5. Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005
  6. Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005.
  7. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model. Clinical Cancer Research 12, 2602-2606, 2006
  8. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. Cell Cycle 8, 870-875, 2009.
  9. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., and Hoffman, R.M. Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhymurium. J. Cell. Biochem. 106, 992-998, 2009.
  10. Tome, Y., Tsuchiya, H., Hayashi, K., Yamauchi, K., Sugimoto, N., Kanaya, F., Tomita, K., and Hoffman, R.M. In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. J. Cell. Biochem. 108, 362-367, 2009. DOI: 10.1002/jcb.22259.
  11. 1 Ongkeko, W.M., Burton, D., Kiang, A., Abhold, E., Kuo, S.Z., Rahimy, E., Yang, M., Hoffman, R.M., Wang-Rodriguez, J., Deftos, L.J. Parathyroid hormone related-protein promotes epithelial-to-mesenchymal transition in prostate cancer. PLoS One 9, e85803, 2014.
  12. Toneri, M., Miwa, S., Zhang, Y., Hu, C., Yano, S., Matsumoto, Y., Bouvet, M., Nakanishi, H., Hoffman, R.M., and Zhao, M. Tumor-targeting Salmonella typhimurium A1-R inhibits human prostate cancer experimental bone metastasis in mouse models. Oncotarget 6, 31335-31343, 2015.
  13. Yano, S., Miwa, S., Kishimoto, H., Urata, Y., Tazawa, H., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Eradication of osteosarcoma by fluorescence-guided surgery with tumor labeling by a killer-reporter adenovirus. J. Orthopaedic Res. 34, 836-844, 2016.

Brain Cancer & Metastasis

  1. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Research 59, 781-786, 1999.
  2. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Research 5, 3549-3559, 1999.
  3. Hoffman, R.M. Green fluorescent protein imaging of tumor cells in mice. Lab Animal 31, 34-41, 2002.
  4. Hoffman, R.M., and Yang, M. Whole-body imaging with fluorescent proteins. Nature Protocols 1, 1429-1438, 2006.
  5. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M. A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis. Cell Proliferation 42, 75-82, 2009.
  6. Hoffman, R.M. Comment re: Preclinical model of spontaneous melanoma metastasis. Cancer Research 69, 719, 2009.
  7. Folkins, C., Shaked, Y., Man, S., Tang, T., Lee, C.R., Zhu, Z., Hoffman, R.M., and Kerbel, R.S. Glioma tumor stem-like cells promote tumor angiogenesis and vasculogenesis via vascular endothelial growth factor and stromal-derived factor 1. Cancer Research 69, 7243-7251, 2009.
  8. Kimura, H., Zhang, L., Zhao, M., Hayashi, K., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M. Targeted therapy of spinal cord glioma with a genetically-modified Salmonella typhimurium. Cell Proliferation 43, 41-48, 2010.
  9. Soda, Y., Marumoto, T., Friedmann-Morvinski, D., Soda, M., Liu, F., Michiue, H., Pastorino, S., Yang, M., Hoffman, R.M., Kesari, S., Verma, I.M. Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc. Natl. Acad. Sci. USA 108, 4274-4280, 2011. Commentary: Hormigo, A., Ding, B-S., and Rafii, S. A target for antiangiogenic therapy: Vascular endothelium derived from glioblastoma. Proc. Natl. Acad. Sci. USA 108, 4271-4272, 2011.
  10. Momiyama, M., Suetsugu, A., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M. Subcellular real-time imaging of the efficacy of temozolomide on cancer cells in the brain of live mice. Anticancer Res. 33, 103-106, 2013.
  11. Momiyama, M., Suetsugu, A., Kimura, H., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M. Dynamic subcellular imaging of cancer cell mitosis in the brain of live mice. Anticancer Res. 33, 1367-1371, 2013.
  12. Momiyama, M., Zhao, M., Kimura, H., Tran, B., Chishima, T., Bouvet, M., Endo, I., and Hoffman R.M. Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model. Cell Cycle 11, 628-632, 2012.
  13. Momiyama, M., Hiroshima, Y., Suetsugu, A., Tome, Y., Mii, S., Yano, S., Bouvet, M., Chishima, T., Endo, I., and Hoffman, R.M. Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice. Anticancer Res. 33, 107-112, 2013.
  14. Rashidi, B., Moossa, A.R., and Hoffman, R.M. Specific route mapping visualized with GFP of single-file streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J. Cell. Biochem 114, 1738-1743, 2013.
  15. Zhang, Y., Miwa, S., Zhang, N., Hoffman, R.M., and Zhao, M. Tumor-targeting Salmonella typhimurium A1-R arrests growth of breast-cancer brain metastasis. Oncotarget 6, 2615-2622, 2015.
  16. Yano, S., Miwa, S., Kishimoto, H., Toneri, M., Hiroshima, Y., Yamamoto, M., Bouvet, M., Urata, Y., Tazawa, H., Kagawa, S., Funiwara, T., and Hoffman, R.M. Experimental curative fluorescence-guided surgery of highly invasive glioblastoma multiforme selectively labeled with a killer-reporter adenovirus. Molecular Therapy 23, 1182-1188, 2015. DOI: 10.1038/mt.2015.63.

Breast Cancer & Metastasis

  1. Miwa, S., Yano, S., Zhang, Y., Matsumoto, Y., Uehara, F., Yamamoto, M.,Hiroshima, Y., Kimura, H., Hayashi, K., Yamamoto, N., Bouvet, M., Tsuchiya, H., Hoffman, R.M., Zhao, M. Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice. Oncotarget 5, 7119-7125, 2014.

Breast

  1. Fu, X., Le, P., and Hoffman, R.M. A metastatic orthotopic-transplant nude-mouse model of human patient breast cancer. Anticancer Res. 13, 901-904, 1993.
  2. Kubota, T., Inoue, S., Furukawa, T., Ishibiki, K., Kitajima, M., Kawamura, E., and Hoffman, R.M. Similarity of serum – Tumor pharmacokinetics of antitumor agents in man and nude mice. Anticancer Res. 13, 1481-1484, 1993.
  3. Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Optically imageable metastatic model of human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002.
  4. John, C.M., Leffler, H., Kahl-Knutsson, B., Svensson, I., Jarvis, G.A. Truncated Galectin-3 Inhibits Tumor Growth and Metastasis in Orthotopic Nude Mouse Model of Human Breast Cancer. Clinical Cancer Research 9, 2374-2383, 2003.
  5. Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R.M., and Tarin, D. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from non-metastatic breast tumors revealed by labeling with green fluorescent protein. Clinical Cancer Res. 9, 3808-3814, 2003.
  6. Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moossa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area. Cancer Res. 66, 303-306, 2006.
  7. Drapeau, C., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., and Schaeffer, D.J. The stem cell mobilizer StemEnhance® does not promote tumor growth in an orthotopic model of human breast cancer. Anticancer Research 29, 443-447, 2009.
  8. Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R. Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo. PLoS One 5, e9712, 2010. DOI: 10.1371/journal.pone.0009712.
  9. Tan, W., Zhang, W., Strasner, A., Grivennikov, S., Cheng, J.Q., Hoffman, R.M., and Karin, M. Tumour-infiltrating regulatory T cells stimulate mammary cancer metastasis through RANKL-RANK signalling. Nature 470, 548-553, 2011.
  10. Zhang, Q., Bindokas, V.P., Shen, J., Fan, H., Hoffman, R.M., Xing, H.R. Time-course imaging of therapeutic functional tumor vascular normalization by anti-angiogenic agents. Mol. Cancer Ther. 10, 1173-1184, 2011.
  11. Hu, M., Zhao, M., An, C., Yang, M., Li, Q., Zhang, Y., Suetsugu, A., Tome, Y., Yano, S., Fu, Y., Hoffman, R.M., and Hu, K. Real-time imaging of apoptosis induction of human breast cancer cells by the traditional Chinese medicinal herb Tubeimu. Anticancer Res. 32, 2509-2514, 2012.
  12. Zhang, Y., Tome, Y., Suetsugu, A., Zhang, L., Zhang, N., Hoffman, R.M., and Zhao M. Determination of the optimal route of administration of Salmonella typhimurium A1-R to target breast cancer in nude mice. Anticancer Research 32, 2501-2508, 2012.
  13. Suetsugu, A., Honma, K., Saji, S., Moriwaki, H., Ochiya, T., and Hoffman, R.M. Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude mouse models. Adv. Drug Deliv. Rev. 65, 383-390, 2013.
  14. Hoffman, R.M. Stromal-cell and cancer-cell exosomes leading the metastatic exodus for the promised niche. Breast Cancer Res. 15, 310-313, 2013.
  15. Wu, J., Ma, R., Cao, H., Wang, Z., Jing, C., Sun, Y., Zhang, Y., Yang, Z., Hoffman, R.M., and Tang, J. Intraoperative imaging of metastatic lymph nodes using a fluorophore-conjugated antibody in a HER2/neu-expressing orthotopic breast cancer mouse model. Anticancer Research 33, 419-424, 2013.
  16. Ke, C-C., Liu, R-S., Suetsugu, A., Kimura, H., Ho, J.H., Lee, O.K., and Hoffman, R.M. In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells. PLoS One 8, e69658, 2013.
  17. Stoletov, K., Strnadel J, Zardouzian, E., Momiyama, M., Park, F.D., Kelber, J.A., Pizzo, D.P., Hoffman, R., VandenBerg, S.R., and Klemke, R.L. Role of connexins in metastatic breast cancer and melanoma brain colonization. J. Cell Sci. 126, 904-913, 2013.
  18. Miwa, S., Yano, S., Zhang, Y., Matsumoto, Y., Uehara, F., Yamamoto, M.,Hiroshima, Y., Kimura, H., Hayashi, K., Yamamoto, N., Bouvet, M., Tsuchiya, H., Hoffman, R.M., Zhao, M. Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice. Oncotarget 5, 7119-7125, 2014.
  19. Zhang, Y., Zhang, N., Hoffman, R.M., and Zhao. M. Surgically-induced multi-organ metastasis in an orthotopic syngeneic imageable model of 4T1 murine breast cancer. Anticancer Res. 35, 4641-4646, 2015.
  20. Kolostova, K., Zhang, Y., Hoffman, R.M., and Bobek, V. Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models. Int. J. Clin. Exp. Med. 9, 7772-7779, 2016.
  21. Yano, S., Takehara, K., Miwa, S., Kishimoto, H., Tazawa, H., Urata, Y., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. In vivo isolation of a highly-aggressive variant of triple-negative human breast cancer variant MDA-MB-231 using serial orthotopic transplantation. Anticancer Research 36, 3817-3820, 2016.
  22. Yano, S., Takehara, K., Miwa, S., Kishimoto, H., Tazawa, H., Urata, Y., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Fluorescence-guided surgery of a highly-metastatic variant of human triple-negative breast cancer targeted with a cancer-specific GFP adenovirus prevents recurrence. Oncotarget 7, 75635-75647, 2016. DOI: 10.18632/oncotarget.12314.
  23. Yano, S., Takehara, K., Kishimoto, H., Tazawa, H., Urata, Y., Kagawa, S., Fujiwara, T., Bouvet, M., Hoffman, R.M. Comparison of tumor recurrence after resection of high- and low-metastatic triple-negative breast cancer in orthotopic nude mouse models. Anticancer Research 37, 57-60, 2017.
  24. Yano, S., Takehara, K., Tazawa, H., Kishimoto, H., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Enhanced metastatic recurrence via lymphatic trafficking of a high-metastatic variant of human triple-negative breast cancer after surgical resection in orthotopic nude mouse models. J. Cell. Biochem. 118, 559-569, 2017.
  25. Yano, S., Takehara, K., Kishimoto, H., Tazawa, H., Urata, Y., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Tumor-targeting adenovirus OBP-401 inhibits primary and metastatic tumor growth of triple-negative breast cancer in orthotopic nude-muse models. Oncotarget 7, 85273-85282, 2016.
  26. Yano, S., Takehara, K., Tazawa, H., Kishimoto, H., Kagawa, S., Fujiwara, T., Hoffman, R.M. Comparison of in vitro invasiveness of high- and low-metastatic triple-negative human breast cancer visualized by color-coded imaging. In Vitro Cell. Dev. Biol.-Animal 53, 96-98, 2017.
  27. Yano, S., Takehara, K., Kishimoto, H., Tazawa, H., Urata, Y., Kagawa, S., Bouvet, M., Fujiwara, T., Hoffman, R.M. OBP-401-GFP telomerase-dependent adenovirus illuminates and kills high-metastatic more effectively than low-metastatic triple-negative breast cancer in vitro. Cancer Gene Therapy 24, 45-47, 2017.

Cervical

  1. Hiroshima, Y., Zhang, Y., Zhang, M., Maawy, A., Mii, S., Yamamoto, M., Uehara, F., Miwa, S., Yano, S., Murakami, T., Momiyama, M., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Murata, T., Endo, I., and Hoffman, R.M. Establishment of a patient-derived orthotopic xenograph (PDOX) model of HER-2-positive cervical cancer expressing the clinical metastatic pattern. PLOS ONE 10, e0117417, 2015.
  2. Murata, T., Mizushima, H., Chinen, I., Moribe, H., Yagi, S., Hoffman, R.M., Kimura, T., Yoshino, K., Ueda, Y., Enomoto, T., and Mekada, E. HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts to support progression of uterine cervical cancers. Cancer Res. 71, 6633-6642, 2011.
  3. Hiroshima, Y., Zhang, Y., Zhao, M., Zhang, N., Murakami, T., Maawy, A., Mii, S., Uehara, F., Yamamoto, M., Miwa, S., Yano, S., Momiyama, M., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R in combination with Trastuzumab eradicates HER-2-positive cervical cancer cells in patient-derived mouse models. PLoS One 10, e0120358, 2015.
  4. Hiroshima, Y., Maawy, A., Zhang, Y., Zhang, N., Murakami, T., Chishima, T., Tanaka, K.., Ichikawa, Y., Bouvet, M., Endo, I., Hoffman, R.M. Patient-derived mouse models of cancer need to be orthotopic in order to evaluate targeted anti-metastatic therapy. Oncotarget 7, 71696-71702, 2016.
  5. Murakami, T., Murata, T., Kawaguchi, K., Kiyuna, T., Igarashi, K., Hwang, H.K., Hiroshima, Y., Hozumi, C., Komatsu, S., Kikuchi, T., Lwin, T.M., Delong, J.C., Miyake, K., Zhang, Y., Tanaka, K., Bouvet, M., Endo, I., Hoffman, R.M. Cervical cancer patient-derived orthotopic xenograft (PDOX) is sensitive to cisplatinum and resistant to nab-paclitaxel. Anticancer Research 37, 61-66, 2017.
  6. Murata, T., Mekada, E., and Hoffman, R.M. Reconstitution of a metastatic-resistant tumor microenvironment with cancer-associated fibroblasts enables metastasis. Cell Cycle 16, 533-535, 2017.

Colon

  1. Fu, X., Besterman, J.M., Monosov, A., and Hoffman, R.M. Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens. Proc. Natl. Acad. Sci. USA 88, 9345-9349, 1991.
  2. Fu, X., Herrera, H., Kubota, T., and Hoffman, R.M. Extensive liver metastasis from human colon cancer in nude and SCID mice after orthotopic onplantation of histologically-intact human colon carcinoma tissue. Anticancer Res. 12, 1395-1398, 1992.
  3. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T.H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M. Immunochemotherapy prevents human colon cancer metastasis after orthotopic onplantation of histologically-intact tumor tissue in nude mice. Anticancer Res. 13, 287-291, 1993.
  4. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M. Early resection of primary orthotopically-growing human colon tumor in nude mouse prevents liver metastasis: further evidence for patient-like hematogenous metastatic route. Anticancer Res. 13, 293-297, 1993.
  5. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Nishibori, H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., and Kitajima, M. A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice. Jpn. J. Surg. 23, 420-423, 1993.
  6. Wang, X., Fu, X., Brown, P.D., Crimmin, M.J., and Hoffman, R.M. Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice. Cancer Res. 54, 4726-4728, 1994.
  7. Togo, S., Shimada, H., Kubota, T., Moossa, A.R., Hoffman, R.M. “Seed” to “soil” is a return trip in metastasis. Anticancer Res. 15, 791-794, 1995.
  8. Togo, S., Shimada, H., Kubota, T., Moossa, A.R., Hoffman, R.M. Host organ specifically determines cancer progression. Cancer Res. 55, 681-684, 1995.
  9. Kuo, T-H., Kubota, T., Watanbe, M., Furukawa, T., Teramoto, T., Ishibiki, K., Kitajimi, M., Moossa, A.R., Penman, S., Hoffman, R.M. Liver colonization competence governs colon cancer metastasis. Proc. Natl. Acad. Sci. USA 92, 12085-12089, 1995.
  10. Togo, S., Wang, X., Shimada, H., Moossa, A.R., and Hoffman, R.M. Cancer seed and soil can be highly selective: Human-patient colon tumor lung metastasis grows in nude mouse lung but not colon or subcutis. Anticancer Res. 15, 795-798, 1995.
  11. Leff, D.N. MetaMouse models colon cancer metastasis with clinical potential. BioWorld Today 7, 1, January 8, 1996.
  12. Murray, G., Duncan, M., O’Neil, P., Melvin, W., Fothergill, J. Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer. Nature Medicine 2, 461-462, 1996.
  13. An, Z., Wang, X., Willmott, N., Chander, S.K., Tickle, S., Docherty, A.J., Mountain, A., Millican, A.T., Morphy, R., Porter, J.R., Epemolu, R.O., Kubota, T., Moossa, A.R., and Hoffman, R.M. Conversion of highly malignant colon cancer from an aggressive to a controlled disease by oral administration of a metalloproteinase inhibitor. Clin & Exp Metastasis 15, 184-195, 1997.
  14. Sun, F-X., Sasson, A.R., Jiang, P., An, Z., Gamagami, R., Li, L., Moossa, A.R., and Hoffman, R.M. An ultra-metastatic model of human colon cancer in nude mice. Clinical & Experimental Metastasis 17, 41-48, 1999.
  15. 15. Sasson, A.R., Gamagami, R., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M. Cimetidine: An inhibitor or promoter of tumor growth? International Journal of Cancer 81, 835-838, 1999.
  16. Rho, Y-S., Lee, K-T., Jung, J-C., Yoon, C., An, Z., Hoffman, R.M., and Chang, S-G. Efficacy of new platinum analog DPPE in an orthotopic nude mouse model of human colon cancer. Anticancer Res. 19, 157-161, 1999.
  17. 17. Rashidi, B., An, Z., Sun, F-X., Sasson, A., Gamagammi, R., Moossa, A.R., Hoffman, R.M. Minimal liver resection strongly stimulates the growth of human colon cancer in the liver of nude mice. Clinical & Experimental Metastasis 17, 497-500, 1999.
  18. Rashidi, B., Sun, F-X., Jiang, P., An, Z., Gamagami, R., Moossa, A.R., and Hoffman, R.M. A nude mouse model of massive liver and lymph node metastasis of human colon cancer. Anticancer Res. 20, 715-722, 2000.
  19. 19. Rashidi, B., An, Z., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Antimetastatic intraoperative chemotherapy of human colon tumors in the livers of nude mice. Clinical Cancer Res. 6, 2464-2468, 2000.
  20. Rashidi, B., Gamagami, R., Sasson, A., Sun, F-X., Geller, J., Moossa, A.R., and Hoffman, R.M. An orthotopic mouse model of remetastasis of human colon cancer liver metastasis. Clinical Cancer Res. 6, 2556-2561, 2000.
  21. Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moossa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area. Cancer Res. 66, 303-306, 2006.
  22. Lukashev, M., LePage, D., Wilson, C., Bailly, V., Garber, E., Lukashin, A., Ngam-ek, A., Zeng, W., Allaire, N., Perrin, S., Xu, X., Szeliga, K., Wortham, K., Kelly, R., Bottiglio, C., Ding, J., Griffith, L., Heaney, G., Silverio, E., Yang, W., Jarpe, M., Fawell, S., Reff, M., Carmillo, A., Miatkowski, K., Amatucci, J., Crowell, T., Prentice, H., Meier, W., Violette, S.M., Mackay, F., Yang, D., Hoffman, R., Browning, J.L. Targeting the lymphotoxin-b receptor with agonist antibodies as a potential cancer therapy. Cancer Rese 66, 9617-9624, 2006.
  23. Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007.
  24. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M. Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007.
  25. Guo, X-N., Rajput, A., Rose, R., Hauser, J., Beko, A., Kuropatwinski, K., LeVea, C., Hoffman, R.M., Brattain, M.G., Wang, J. Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007.
  26. Rajput, A., Dominguez San Martin, I., Rose, R., Beko, A., Levea, C., Sharratt, E., Mazurchuk, R., Hoffman, R.M., Brattain, M.G., and Wang, J. Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008.
  27. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., Yamashita, S. In vitro/in vivo biorecognition of lectin-immobilized fluorescent nanospheres for human colorectal cancer cells. Journal of Controlled Release 134, 2-10, 2009.
  28. Ma, H., Das, T., Pereira, S., Yang, Z., Zhao, M., Mukerji, P., and Hoffman, R.M. Efficacy of dietary antioxidants combined with a chemotherapeutic agent on human colon cancer progression in a fluorescent orthotopic mouse model. Anticancer Research 29, 2421-2426, 2009.
  29. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K.I., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., and Yamashtia, S. Detection of early colorectal cancer imaged with peanut agglutinin-immobilized fluorescent nanospheres having surface poly(N-vinylacetamide) chains. Eur. J. Pharma. Biopharma. 74, 451-460, 2010.
  30. Ishihara, Y., Matsunaga, K., Iijima, H., Hasegawa, G., Suzuki, T., Sato, A., Kobayashi, T., Yang, M., and Hoffman, R.M. The combination of 5-FU, leucovorin and CPT-11 (FOLFIRI) prolongs survival through inhibition of metastasis in an orthotopic model of colon cancer. Anticancer Res. 30, 403-408, 2010.
  31. Suetsugu, A., Osawa, Y., Nagaki, M., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Imaging the recruitment of cancer-associated fibroblasts by liver-metastatic colon cancer. J. Cell. Biochem. 112, 949-953, 2011.
  32. Ma, H., Li, X., Yang, Z., Okuno, S., Kawaguchi, T., Yagi, S., Bouvet, M, and Hoffman, R.M. High antimetastatic efficacy of MEN4901/T-0128, a novel camptothecin carboxymethyldextran conjugate. J. Surg. Res. 171, 684-690, 2011.
  33. Kishimoto, H., Aki, R., Urata, Y., Bouvet, M., Momiyama, M., Tanaka, N., Fujiwara, T., Hoffman, R.M. Tumor-selective adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection. Cell Cycle 10, 2737-2741, 2011.
  34. Sakuma, S., Yamashita, S., Hiwatari, K., Hoffman, R.M., and Pham, W. Lectin-immobilized fluorescent nanospheres for targeting to colorectal cancer from a physicochemical perspective. Curr. Drug Discov. Technol. 8, 367-378, 2011.
  35. Momiyama, M., Kumamoto, T., Suetsugu, A., Kishimoto, H., Chishima, T., Tanaka, K., Akiyama, H., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M. Major liver resection stimulates stromal recruitment and metastasis compared with repeated minor resection. J. Surg. Res. 178, 280-287, 2012.
  36. Metildi, C.A., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet, M. Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model. J. Surg. Res. 179, 87-93, 2013.
  37. Metildi, C.A., Kaushal, S., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Fluorescently-labeled chimeric anti-CEA antibody improves detection and resection of human colon cancer in a patient-derived orthotopic xenograft (PDOX) nude mouse model. J Surg. Oncol. 109, 451-458, 2014.
  38. Maawy, A., Hiroshima, Y., Zhang, Y., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Polyethylene glycol (PEG) conjugations of chimeric anti-carcinoembryonic antigen (CEA) labeled with near infrared (NIR) dyes enhances imaging of liver metastases in a nude-mouse model of human colon cancer. PLoS One 9, e97965, 2014.
  39. Kikuchi, S., Kishimoto, H., Tazawa, H., Hashimoto, Y., Kuroda, S., Nishizaki, M., Nagasaka, T., Shirakawa, Y., Kagawa, S., Urata, Y., Hoffman, R.M., and Fujiwara, T. Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer. Molecular Therapy 23, 501-509, 2015. DOI: 10.1038/mt.2014.244.
  40. Kitamura, T., Sakuma, S., Shimosato, M., Higashino, H., Masaoka, Y., Kataoka, M., Yamashita, S., Hiwatari, K., Kumagai, H., Morimoto, N., Koike, S., Tobita, E., Hoffman, R.M., Gore, J.C., and Pham, W. Specificity of lectin-immobilized fluorescent nanospheres for colorectal tumors in a mouse model which better resembles the clinical disease. Contrast Media Mol. Imaging 10, 135-143, 2015. DOI: 10.2002/cmmi.1609.
  41. Murakami, T., Hiroshima, Y., Zhao, M., Zhang, Y., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Adjuvant treatment with tumor-targeting Salmonella typhimurium A1-R reduces recurrence and increases survival after liver metastasis resection in an orthotopic nude mouse model. Oncotarget 6, 41856-41862, 2015.
  42. Park, J., Murakami, T., Lee, J., Zhang, Y., Hoffman, R.M., Bouvet, M. Fluorescent-antibody targeting of insulin-like growth factor-1 receptor visualizes metastatic human colon cancer in orthotopic mouse models. PLoS One 11, e0146504, 2016.
  43. Yano, S., Takehara, K., Miwa, S., Kishimoto, H., Hiroshima, Y., Murakami, T., Urata, Y., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Improved resection and outcome of colon-cancer liver metastasis with fluorescence-guided surgery using in situ GFP labeling with a telomerase-dependent adenovirus in an orthotopic mouse model. PLoS One 11, e0148760, 2016.
  44. Sun, J., Ling, B., Xu, X., Ma, R., Li, G., Cao, X., Ling, W., Yang, Z., Hoffman, R.M., and Lu, J. Decreased expression of tumor-suppressor gene LKB1 correlates with poor prognosis in human gastric cancer. Anticancer Research 36, 869-875, 2016.
  45. Hiroshima, Y., Matsuo, K., Kawaguchi, D., Kikuchi, Y., Endo, I., Koda, K., Togo, S., Hoffman, R.M., and Tanaka, K. Modified liver hanging maneuver for en-bloc right-sided hepatectomy combined with total caudate lobectomy for colon-cancer liver metastasis and hepatocellular carcinoma. Anticancer Research 36, 1729-1735, 2016.
  46. Dai, X., Liu, D., Liu, M., Zhang, X., Wang, W., Jin, F., Qian, Y., Wang, X., Zhao, J., Wu, Y., Xiong, F., Chang, N., Sun Y., Yang, Z., Hoffman, R.M., Liu, Y. Anti-metastatic efficacy of traditional Chinese medicine (TCM) ginsenoside conjugated to an VEFGR-3 antibody on human gastric cancer in an orthotopic mouse model. Anticancer Res. 37, 979-986, 2017.
  47. Kawaguchi, K., Murakami, T., Suetsugu, A., Kiyuna, T., Igarashi, K., Hiroshima, Y., Zhao, M., Zhang, Y., Bouvet, M., Clary, B., Unno, M., Hoffman, R.M. High-efficacy targeting of colon-cancer liver metastasis with Salmonella typhimurium A1-R via intra-portal-vein injection in orthotopic nude-mouse models. Oncotarget 8, 19065-19073, 2017.
  48. Hiroshima, Y., Lwin, T.M., Murakami, T., Maawy, A.A., Kuniya, T., Chishima, T., Endo, I., Clary, B.M., Hoffman, R.M., Bouvet, M. Effective fluorescence guided surgery of liver metastasis using fluorescent anti-CEA antibody. J. Surg. Onc. 114, 951-958, 2016.

Esophageal

  1. Gros, S.J., Dohrmann, T., Peldschus, K., Schurr, P.G., Kaifi, J.T., Kalinina, T., Reichelt, U., Mann, O., Strate, T.G., Adam, G., Hoffman, R.M., and Izbicki, J.R. Complementary use of fluorescence and magnetic resonance imaging of metastatic esophageal cancer in a novel orthotopic mouse model. International Journal of Cancer 126, 2671-2681, 2010.
  2. Gros, S., Kurschat, N., Dohrmann, T., Reichelt, U., Dancau, A-M., Peldschus, K., Adam, G., Hoffman, R.M., Izbicki, J.R., and Kaifi, J.T. Effective therapeutic targeting of the overexpressed HER-2 receptor in a highly metastatic orthotopic model of esophageal carcinoma. Molecular Cancer Therapeutics 9, 2037-2045, 2010.
  3. Gros, S.J., Dohrmann, T., Kaifi, J.T., Bouvet, M., Hoffman, R.M., Izbicki, J.R. Orthotopic fluorescent peritoneal carcinomatosis model of esophageal cancer. Anticancer Research 30, 3933-3938, 2010.
  4. Gros., S.J., Kurschat, N., Drenckhan, A., Dohrmann, T., Forberich, E., Effenberger, K., Reichelt, U., Hoffman, R.M., Pantel, K., Kaifi, J.T., and Izbicki, J.R. Involvement of CXCR-4 chemokine receptor in metastastic HER2-positive esophageal cancer. PLoS One 7(10), e47287, 2012.
  5. Hu, T., Qi, H., Li, P., Zhao, G., Ma, Y., Hao, Q., Gao, C., Zhang Y., Wang, C., Yang, M., Hoffman, R.M., Chen, P., and Dong, Z. Comparison of GFP-expressing imageable mouse models of human esophageal squamous cell carcinoma established in various anatomical sites. Anticancer Res. 35, 4655-4664, 2015.

Fibrosarcoma

  1. Uehara, F., Hiroshima, Y., Miwa, S., Tome, Y., Yano, S., Yamamoto, M., Matsumoto, Y., Maehara, H., Tanaka, K., Bouvet, M., Kanaya, F., Hoffman, R.M. Fluorescence-guided surgery of retroperitoneal-implanted human fibrosarcoma in nude mice delays or eliminates tumor recurrence and increases survival compared to bright-light surgery. PLoS One 10, e0116865, 2015.

Gene Therapy

  1. Miki, K., Xu, M., An, Z., Wang, X., Yang, M., Al-Refaie, W., Sun, X., Baranov, E., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Survival efficacy of the combination of the methioninase gene and methioninase in a lung cancer orthotopic model. Cancer Gene Therapy 7, 332-338, 2000.
  2. Miki, K., Al-Refaie, W., Xu, M., Jiang, P., Tan, Y., Bouvet, M., Zhao, M., Gupta, A., Chishima, T., Shimada, H., Makuuchi, M., Moossa, A.R., and Hoffman, R.M. Methioninase gene therapy of human cancer cells is synergistic with recombinant methioninase treatment. Cancer Res. 60, 2696-2702, 2000.
  3. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.
  4. Djeha, A.H., Thomson, T.A., Leung, H., Searle, P.F., Young, L.S., Kerr, D.J., Harris, P.A., Mountain, A., and Wrighton, C.J. Combined adenovirus-mediated nitroreductase gene delivery and CB1954 treatment: A well-tolerated therapy for established solid tumors. Molecular Therapy 3, 233-240, 2001.
  5. Chen, C.T., Lin, J., Li, Q., Phipps, S.S., Jakubczak, J.L., Stewart, D.A., Skripchenko, Y., Forry-Schaudies, S., Wood, J., Schnell, C., and Hallenbeck, P.L. Antiangiogenic gene therapy for cancer via systemic administration of adenoviral vectors expressing secretable endostatin. Human Gene Therapy 11, 1983-1996, 2000.
  6. Miki, K, Xu, M., Gupta, A., Ba, Y., Tan, Y., Al-Refaie, W., Bouvet, M., Makuuchi, M., Moossa, A.R., and Hoffman, R.M. Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate. Cancer Research 61, 6805-6810, 2001.
  7. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H. VEGF receptor antisense therapy inhibits angiogenesis and peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy 9, 197-201, 2002.
  8. Pirocanac, E.C., Nassirpour, R., Yang, M., Wang, J-W., Nardin, S.R., Gu, J., Fang, B., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Bax-induction gene therapy of pancreatic cancer. J. Surg. Res. 106, 346-351, 2002.
  9. Hoffman, R.M. Immune reactions in skin and hair follicle gene therapy. Molecular Therapy 7, 294-295, 2003.
  10. Katz, M.H., Spivack, D.E., Takimoto, S., Fang, B., Burton, D.W., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Gene therapy of pancreatic cancer with green fluorescent protein and tumor necrosis factor-related apoptosis-inducing ligand fusion gene expression driven by a human telomerase reverse transcriptase promoter. Annals of Surgical Oncology 10, 762-772, 2003.

Head & Neck

  1. Lee, S.E., Bairstow, S.F., Werling, J.O., Chaubal, M.V., Lin, L., Murphy, M.A., Diorio, J. P., Gass, J., Rabinow, B., Wang, X., Zhang, Y., Yang, Z., and Hoffman, R.M. Paclitaxel nanosuspensions for targeted chemotherapy – nanosuspension preparation, characterization, and use. Pharm. Dev. Technol. 19, 438-453, 2014.

Kidney

  1. An, Z., Jiang, P., Wang, X., Moossa, A.R., and Hoffman, R.M. Development of a high metastatic orthotopic model of human renal cell carcinoma in nude mice: Benefits of fragment implantation compared to cell-suspension injection. Clinical & Experimental Metastasis 17, 265-270, 1999.

Liver

  1. Sun, F.X., Tang, Z.Y., Liu, K.D., Ye, S.L., Xue, Q., Gao, D.M., Ma, Z.C. Establishment of a metastatic model of human hepato-cellular carcinoma in nude mice via orthotopic implantation of histologically intact tissues. Int. J. Cancer 66, 239-243, 1996.
  2. Sun, F-X., Tang, Z-Y., Liu, K-D., Xue, Q., Gao, D-M., Yu, Y-Q., Zhou, X-D., Ma, Z-C. Metastatic models of human liver cancer in nude mice orthotopically constructed by using histologically intact patient specimens. J. Cancer Res. Clin. Oncol. 122, 397-402, 1996.
  3. Rashidi, B., An, Z., Sun, F-X, Li, X-M., Tang, Z.Y., Moossa, A.R., and Hoffman, R.M. Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice. Int. J. Cancer 91, 231-235, 2001.
  4. Wu, M., Mazurchuk, R., Chaudhary, N.D., Spernyak, J., Veith, J., Pera, P., Greco, W., Hoffman, R.M., Kobayashi, T., and Bernacki, R.J. High-Resolution Magnetic Resonance Imaging of the Efficacy of the Cytosine Analogue 1-[2-C-Cyano-2-deoxy-b-D-arabino-pentofuranosyl]-N4-palmitoyl Cytosine (CS-682) in a Liver-Metastasis Athymic Nude Mouse Model. Cancer Res. 63, 2477-2482, 2003.
  5. Suetsugu, S., Osawa, Y., Nagaki, M., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M. Simultaneous color-coded imaging to distinguish cancer “stem-like” and non-stem cells in the same tumor. J. Cell. Biochem. 111, 1035-1041, 2010.
  6. Wang, M., Zhang, X., Xiong, X., Yang, Z., Sun, Y., Yang, Z., Hoffman, R.M., and Liu, Y. Efficacy of the Chinese Traditional Medicinal Herb Celastrus orbiculatus Thunb on human hepatocellular carcinoma in an orthotopic fluorescent nude mouse model. Anticancer Res. 32, 1213-1220, 2012.
  7. Momiyama, M., Kumamoto, T., Suetsugu, A., Kishimoto, H., Chishima, T., Tanaka, K., Akiyama, H., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M. Major liver resection stimulates stromal recruitment and metastasis compared with repeated minor resection. J. Surg. Res. 178, 280-287, 2012.
  8. He, G., Dhar, D., Nakagawa, H., Font-Burgada, J., Ogata, H., Jiang, Y., Shalapour, S., Seki, E., Yost, S.E., Jepsen, K., Frazer, K.A., Harismendy, O., Hatziapostolou, M.,Iliopoulos, D., Suetsugu, A., Hoffman, R.M., Tateishi, R., Koike, K., Karin, M. Identification of Liver Cancer Progenitors Whose Malignant Progression Depends on Autocrine IL-6 Signaling. Cell 155, 384-396, 2013.
  9. Chen, P., Hu, T., Liang, Y., Jiang, Y., Pan, Y., Li, C., Zhang, P., Wei, D., Li, P., Jeong, L.S., Chu, Y., Qi, H., Yang, M., Hoffman, R.M., Dong, Z., and Jia, L. Synergistic inhibition of autophagy and neddylation pathways as a novel therapeutic approach for targeting liver cancer. Oncotarget 6, 9002-9017, 2015.
  10. Suetsugu, A., Hiroshima, Y., Matsumoto, T., Hasagawa, K., Nakamura, M., Shimizu, M., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Recruitment of cancer-associated fibroblasts and blood vessels by orthotopic liver tumors imaged in red fluorescent protein (RFP) transgenic nude mice. Anticancer Research 35, 5821-5825, 2015.
  11. Hiroshima, Y., Matsuo, K., Kawaguchi, D., Kikuchi, Y., Endo, I., Koda, K., Togo, S., Hoffman, R.M., and Tanaka, K. Modified liver hanging maneuver for en-bloc right-sided hepatectomy combined with total caudate lobectomy for colon-cancer liver metastasis and hepatocellular carcinoma. Anticancer Research 36, 1729-1735, 2016.
  12. Wang, M., Zhang, X., Xiong, X., Yang, Z., Li, P., Wang, J., Sun, Y., Yang, Z., and Hoffman, R.M. Bone marrow mesenchymnal stem cells reverse liver damage in a carbon tetrachloride-induced mouse model of chronic liver injury. In vivo 30, 187-194, 2016.

Lung

  1. Wang, X., Fu, X., and Hoffman, R.M. A new patient-like metastatic model of human lung cancer constructed orthotopically with intact tissue via thoracotomy in immunodeficient mice. Int. J. Cancer 51, 992-995, 1992.
  2. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Kase, S., Tanino, H., Nishibori, K., Saikawa, Y., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M. Orthotopic reconstitution of human small-cell lung carcinoma after intravenous transplantation in SCID mice. Anticancer Res. 12, 1407-1410, 1992.
  3. Wang, X., Fu, X., and Hoffman, R.M. A patient-like metastasizing model of human lung adenocarcinoma constructed via thoracotomy in nude mice. Anticancer Res. 12, 1399-1402, 1992.
  4. Wang, X., Fu, X., Kubota, T., and Hoffman, R.M. A new patient-like metastatic model of human small-cell lung cancer constructed orthotopically with intact tissue via thoracotomy in nude mice. Anticancer Res. 12, 1403-1406, 1992.
  5. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Kase, S., Tanino, H., Saikawa, Y., Ishibiki, K., Kitajima, M., and Hoffman, R.M. Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice: the importance of orthotopic models to obtain relevant drug evaluation data. Anticancer Res. 13, 627-630, 1993.
  6. Astoul, P., Wang, X., and Hoffman, R.M. "Patient-like" nude- and SCID-mouse models of human lung and pleural cancer (Review). Int. J. Oncology 3, 713-718, 1993.
  7. Astoul, P., Colt, H.G., Wang, X., and Hoffman, R.M. A “patient-like” nude mouse model of parietal pleural human lung adenocarcinoma. Anticancer Res. 14, 85-92, 1994.
  8. Astoul, P., Colt, H.G., Wang, X., Boutin, C. and Hoffman, R.M. “Patient-like” nude mouse metastatic model of advanced human pleural cancer. Journal of Cellular Biochemistry 56, 9-15, 1994.
  9. Astoul, P., Wang, X., Colt, H.G., Boutin, C., and Hoffman, R.M. A patient-like human malignant pleural mesothelioma nude-mouse model. Oncology Reports 3, 483-487, 1996.
  10. Colt, H.G., Astoul, P., Wang, X., Yi, E.S., Boutin, C., and Hoffman, R.M. Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model. Anticancer Res. 16, 633-639, 1996.
  11. An, Z., Wang, X., Astoul, P., Danays, T., Moossa, A.R., and Hoffman, R.M. Interferon gamma is highly effective against orthotopically-implanted human pleural adenocarcinoma in nude mice. Anticancer Res. 16, 2545-2551, 1996.
  12. Olbina, G., Cieslak, D., Ruzdijic, S., Esler, C., An, Z., Wang, X., Hoffman, R., Seifert, W., and Pietrzkowski, Z. Reversible inhibition of IL-8 receptor B mRNA expression and proliferation in non-small cell lung cancer by antisense oligonucleotides. Anticancer Res. 16, 3525-3530, 1996.
  13. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression. Cancer Res. 58, 4217-4221, 1998.Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M. A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model. Clinical & Experimental Metastasis 18, 57-60, 2000.
  14. Hoffman, Robert M. Metastatic Orthotopic Mouse Models of Lung Cancer. In: Methods in Molecular Medicine. Lung Cancer, Vol. 1: Molecular Pathology Methods and Reviews, pp. 457-464. Driscoll, B., ed. Totowa, NJ: Humana Press, 2003.
  15. Yamamoto, N., Yang, M., Jiang, P., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases. Clin. Exp. Metastasis 20, 181-185, 2003.
  16. Bobek, V., Boubelik, M., Fiserova, A., L’uptovcova, M., Vannucci, L., Kacprzak, G., Kolodzej, J., Majewski, A.M., and Hoffman, R.M. Anticoagulant drugs increase natural killer cell activity in lung cancer. Lung Cancer 47, 215-223, 2005.
  17. Hoffman, R.M. Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007.
  18. Zhang, C., Lv, F., Zhou, L., Li, X., Wu, X-X., and Hoffman, R.M. Effect of verapamil on the expression of EGFR and NM23 in A549 human lung cancer cells. Anticancer Res. 29, 27-32, 2009.
  19. Kimura, H., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Real-time imaging of single cancer-cell dynamics of lung metastasis. J. Cell. Biochem. 109, 58-64, 2010.
  20. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., Gurlich, R., and Hoffman, R.M. Tail spontaneous metastatic mouse model: Comparison of metastatic potential of orthotopic and heterotopic models imaged by GFP and RFP protein. In Vivo 25, 849-852, 2011.
  21. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., and Hoffman, R.M. Development of new spontaneous metastatic heterotopic model of Lewis lung carcinoma imaged by GFP Expression. Cancer Invest. 29, 692-695, 2011.
  22. Zhang, Y., Zhang, N., Su, S., Hoffman, R.M., Zhao, M. Salmonella typhimurium A1-R tumor targeting in immunocompetent mice is enhanced by a Traditional Chinese Medicine herbal mixture. Anticancer Res. 33, 1837-1843, 2013.
  23. Rashidi, B., Moossa, A.R., and Hoffman, R.M. Specific route mapping visualized with GFP of single-file streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J. Cell. Biochem 114, 1738-1743, 2013.
  24. Li, L., Wang, M., Yu, G., Chen P., Li, H., Wei, D., Zhu, J., Xie, L., Jia H., Shi, J., Li, C., Yao, W., Wang, Y., Gao, Q., Jeong, L.S., Lee, H.W., Yu, J., Hu, F., Mei, J., Wang, P., Chu, Y., Qi, H., Yang, M., Dong, Z., Sun, Y., Hoffman, R.M., and Jia, L. Overactivated neddylation pathway as a therapeutic target in lung cancer. J. Natl. Cancer Inst. 106(6), dju083, 2014.
  25. Kolostova, K., Zhang, Y., Hoffman, R.M., and Bobek, V. In vitro culture and characterization of human lung cancer circulating tumor cells isolated by size exclusion from an orthotopic nude-mouse model expressing fluorescent protein. J. Fluoresc. 24, 1531-1536, 2014.
  26. Zhang, L., Wu, C., Zhang, Y., Liu, F., Wang, X., Zhao, M., and Hoffman, R.M. Comparison of efficacy and toxicity of Traditional Chinese Medicine (TCM) herbal mixture LQ and conventional chemotherapy on lung cancer metastasis and survival in mouse models. PLoS One 9, e109814, 2014.
  27. Yano, S., Zhang, Y., Miwa, S., Kishimoto, H., Urata, Y., Bouvet, M., Kagawa, S., Fujiwara, T., and Hoffman, R.M. Precise navigation surgery of tumours in the lung in mouse models enabled by in situ fluorescence labelling with a killer-reporter adenovirus. BMJ Open Respiratory Research 2, e000096, 2015.
  28. Zhang, Y., Zhang, N., Zhao, M., and Hoffman, R.M. Real-time non-invasive spectral imaging of orthotopic red fluorescent protein-expressing lung tumor growth in nude mice. Anticancer Res. 35, 3755-3759, 2015.
  29. Zhang, Y., Zhang, N., Zhao, M., and Hoffman, R.M. Comparison of the selective targeting efficacy of Salmonella typhimurium A1-R and VNP20009 on the Lewis lung carcinoma in nude mice. Oncotarget 6, 14625-14631, 2015.

Lymphoma

  1. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M. and Lowe, S.W. Dissecting p53 tumor suppressor functions in vivo. Cancer Cell 1, 289-298, 2002.
  2. Schmitt, C.A., Yang, M., Fridman, J.S., Baranov, E., Hoffman, R.M., and Lowe, S.W. A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy. Cell 109, 335–346, 2002.
  3. Matsumoto, T., Suetsugu, A., Shibata, Y., Nakamura, N., Aoki, H., Kunisada, T., Tsurumi, H., Shimizu, M., and Hoffman, R.M. A color-coded imageable syngeneic mouse model of stromal-cell recruitment by metastatic lymphoma. Anticancer Res 35, 4647-4654, 2015.
  4. Matsumoto, T., Suetsugu, A., Hasegawa, K., Nakamura, M., Aoki, H., Kunisada, T., Tsurumi, H., Shimizu, M., and Hoffman, R.M. Color-coded imaging of syngeneic orthotopic malignant lymphoma interacting with host stromal cells during metastasis. Anticancer Research 36, 1473-1478, 2016.

Melanoma

  1. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Res. 5, 3549-3559, 1999.
  2. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000.
  3. Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M. Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells. Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.
  4. Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Hair follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin. Cancer Res. 65, 2337-2343. 2005.
  5. Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R. Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo. PLoS One 5, e9712, 2010. DOI: 10.1371/journal.pone.0009712.
  6. Tsai, M-H., Aki, R., Amoh, Y., Hoffman, R.M., Katsuoka, K., Kimura, H., Lee, C., and Chang, C-H. GFP-fluorescence-guided UVC irradiation inhibits melanoma growth and angiogenesis in nude mice. Anticancer Research 30, 3291-3294, 2010.
  7. Bobek, V., Kolostova, K., Pinterova, D., Kacprzak, G., Adamiak, J., Kolodzej, J., Boubelik, M., Kubecova, M., and Hoffman, R.M. A clinically relevant, syngeneic model of spontaneous, highly-metastatic B16 mouse melanoma. Anticancer Research 30, 4799-4804, 2010.
  8. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., Gurlich, R., and Hoffman, R.M. Tail spontaneous metastatic mouse model: Comparison of metastatic potential of orthotopic and heterotopic models imaged by GFP and RFP protein. In Vivo 25, 849-852, 2011.
  9. Binder, D.C., Engels, B., Arina, A., Yu, P., Stauch, J.M., Fu, Y-X, Karrison, T., Burnette, B., Idel, C., Zhao, M., Hoffman, R.M., Munn, D.H., Rowley, D.A., and Schreiber, H. Antigen-specific bacterial vaccine combined with anti-PD-L1 rescues dysfunctional endogenous T cells to reject long-established cancer. Cancer Immunol. Res., 1, 123-133, 2013.
  10. Aki, R., Amoh, Y., Bouvet, M., Katsuoka, K., and Hoffman, R.M. Color-coded fluorescence imaging of lymph-node metastasis, angiogenesis, and its drug-induced inhibition. J. Cell. Biochem. 115, 457-463, 2014.
  11. Stoletov, K., Strnadel J, Zardouzian, E., Momiyama, M., Park, F.D., Kelber, J.A., Pizzo, D.P., Hoffman, R., VandenBerg, S.R., and Klemke, R.L. Role of connexins in metastatic breast cancer and melanoma brain colonization. J. Cell Sci. 126, 904-913, 2013.
  12. Yamamoto, M., Zhao, M., Hiroshima, Y., Zhang, Y., Shurell, E., Eilber, F.C., Bouvet, M., Noda, M.,Hoffman, R.M. Efficacy of tumor-targeting Salmonella typhimurium A1-R on a melanoma patient-derived orthotopic xenograft (PDOX) nude-mouse model. PLoS One 11, e0160882, 2016.
  13. Kawaguchi, K., Murakami, T., Chmielowski, B., Igarashi, K., Kiyuna, T., Unno, M., Nelson, S.D., Russell, T.A., Dry, S.M., Li, Y., Eilber, F.C., and Hoffman, R.M. Vemurafenib-resistant BRAF-V600E mutated melanoma is regressed by MEK targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model. Oncotarget 7, 71737-71743, 2016.
  14. Kawaguchi, K., Igarashi, K., Murakami, T., Chmiewloski, B., Kiyuna, T., Zhao, M., Zhang, Y., Singh, A., Unno, M., Nelson, S.D., Russell, T., Dry, S.M., Li, Y., Eilber, F.C., Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R combined with Temozolomide regresses malignant melanoma with a BRAF-V600 mutation in a patient-derived orthotopic xenograft (PDOX) model. Oncotarget 7, 85929-85936, 2016.
  15. Kawaguchi, K., Murakami, T., Chmielowski, B., Igarashi, K., Kiyuna, T., Unno, M., Nelson, S.D., Russell, T.A., Dry, S.M., Li, Y., Eilber, F.C., and Hoffman, R.M. Vemurafenib-resistant BRAF-V600E mutated melanoma is regressed by MEK targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model. Oncotarget 7, 71737-71743, 2016.
  16. Kawaguchi, K., Igarashi, K., Murakami, T., Chmiewloski, B., Kiyuna, T., Zhao, M., Zhang, Y., Singh, A., Unno, M., Nelson, S.D., Russell, T., Dry, S.M., Li, Y., Eilber, F.C., Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R combined with Temozolomide regresses malignant melanoma with a BRAF-V600 mutation in a patient-derived orthotopic xenograft (PDOX) model. Oncotarget 7, 85929-85936, 2016.

Multiple Myeloma

  1. Mitsiades, C.S., Mitsiades, N.S., Bronson, R.T., Chauhan, D., Munshi, N., Treon, S.P., Maxwell, C.A., Pilarski, L., Hideshima, T., Hoffman, R.M., and Anderson, K.C. Fluorescence imaging of multiple myeloma cells in a clinically relevant SCID/NOD in vivo model: biologic and clinical implications. Cancer Research 63, 6689-6696, 2003.

Nasopharyngeal Carcinoma

  1. Liu, T., Ding, Y., Xie, W., Li, Z., Bai, X., Li, X., Fang, W., Ren, C., Wang, S., Hoffman, R.M., and Yao, K. An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin. Cancer Res. 13, 3960-3967, 2007.

Ovarian

  1. Fu, X., and Hoffman, R.M. Human ovarian carcinoma metastatic models constructed in nude mice by orthotopic transplantation of histologically-intact patient specimens. Anticancer Res. 13, 283-286, 1993.
  2. Astoul, P., Colt, H.G., Wang, X., and Hoffman, R.M. Metastatic human pleural ovarian cancer model constructed by orthotopic implantation of fresh histologically-intact patient carcinoma in nude mice. Anticancer Res. 13, 1999-2002, 1993.
  3. Kiguchi, K., Kubota, T., Aoki, D., Udagawa, Y., Yamanouchi, S., Saga, M., Amemiya, A., Nozawa, S., Moossa, A.R., and Hoffman, R.M. A patient-like orthotopic implantation nude mouse model of highly metastatic human ovarian cancer. Clinical & Experimental Metastasis 16, 751-756, 1998.
  4. Steinkamp, M.P., Winner, K.K., Davies, S., Muller, C., Zhang, Y., Hoffman, R.M., Shirinifard, A., Moses, M., Jiang, Y., and Wilson, B.S. Ovarian tumor attachment, invasion, and vascularization reflect unique microenvironments in the peritoneum: insights from xenograft and mathematical models. Frontiers in Oncology, published online, 2013. DOI: 10.3399/fonc.2013.00097.
  5. Lee, S.E., Bairstow, S.F., Werling, J.O., Chaubal, M.V., Lin, L., Murphy, M.A., Diorio, J. P., Gass, J., Rabinow, B., Wang, X., Zhang, Y., Yang, Z., and Hoffman, R.M. Paclitaxel nanosuspensions for targeted chemotherapy – nanosuspension preparation, characterization, and use. Pharm. Dev.Technol. 19, 438-453, 2014.
  6. Matsumoto, Y., Miwa, S., Zhang, Y., Hiroshima, Y., Yano, S., Uehara, F., Yamamoto, M., Toneri, M., Bouvet, M., Matsubara, H., Hoffman, R.M., and Zhao, M. Efficacy of tumor-targeting Salmonella typhimurium A1-R on nude mouse models of metastatic and disseminated human ovarian cancer. J. Cell. Biochem. 115, 1996-2003, 2014.
  7. Matsumoto, Y., Miwa, S., Zhang, Y., Zhao, M., Yano, S., Uehara, F., Yamamoto, M., Hiroshima, Y., Toneri, M., Bouvet, M., Matsubara, H., Tsuchiya, H., and Hoffman, R.M. Intraperitoneal administration of tumor-targeting Salmonella typhimurium A1-R inhibits disseminated human ovarian cancer and extends survival in nude mice. Oncotarget 6, 11369-11377, 2015.
  8. Yin, G., Tang, D., Dai, J., Liu, M., Wu, M., Sun, Y., Yang, Z., Hoffman, R.M., Li, L., Zhang, S., and Guo, X. Combination efficacy of Astragalus membranaceus and Curcuma wenyujin at different stages of tumor progression in an orthotopic nude mouse model of metastatic human ovarian cancer. Anticancer Res. 35, 3193-3208, 2015.

Pancreas

  1. Fu, X., Guadagni, F., and Hoffman, R.M. A metastatic nude-mouse model of human pancreatic cancer constructed orthotopically from histologically intact patient specimens. Proc. Natl. Acad. Sci. USA 89, 5645-5649, 1992.
  2. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M. A novel "patient-like" treatment model of human pancreatic cancer constructed using orthotopic transplantation of histologically intact human tumor tissue in nude mice. Cancer Res. 53, 3070-3072, 1993.
  3. An, Z., Wang, X., Kubota, T., Moossa, A.R., Hoffman, R.M. A clinical nude mouse metastatic model for highly malignant human pancreatic cancer. Anticancer Res. 16, 627-631, 1996.
  4. Tomikawa, M., Kubota, T., Matsuzaki, S.W., Takahasi, S., Kitajima, M., Moossa, A.R., and Hoffman, R.M. Mitomycin C and cisplatin increase survival in a human pancreatic cancer metastatic model. Anticancer Res. 17, 3623-3626, 1997.
  5. Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E., Moossa, A.R., Hoffman, R.M. Chronologically-specific metastatic targeting of human pancreatic tumors in orthotopic models. Clinical & Experimental Metastasis 18, 213-218, 2000.
  6. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., Hoffman, R.M. Anti-metastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clin & Exp Metastasis 18, 379-384, 2001.
  7. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M. Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model. Cancer Research 62, 1534-1540, 2002.
  8. Bouvet, M., Nardin, S.R., Burton, D.W., Lee, N.C., Yang, M., Wang, X., Baranov, E., Behling, C., Moossa, A.R., Hoffman, R.M., Deftos L.J. Parathyroid hormone-related protein as a novel tumor marker in pancreatic adenocarcinoma. Pancreas 24, 284-290, 2002.
  9. Sun, F-X., Tohgo, A., Bouvet, M., Yagi, S., Nassirpour, R., Moossa, A.R., and Hoffman, R.M. Efficacy of camptothecin analog DX-8951f (Exatecan Mesylate) on human pancreatic cancer in an orthotopic metastatic model. Cancer Research 63, 80-85, 2003.
  10. Katz, M., Takimoto, S., Spivack, D., Moossa, A.R., Hoffman, R.M., Bouvet, M. A novel red fluorescent protein orthotopic pancreatic cancer model for the preclinical evaluation of chemotherapeutics. J. Surg. Res. 113, 151-160, 2003.
  11. Katz, M.H., Bouvet, M., Takimoto, S., Spivack, D., Moossa, A.R., Hoffman, R.M. Selective antimetastatic activity of cytosine analog CS-682 in a red fluorescent protein orthotopic model of pancreatic cancer. Cancer Res. 63, 5521-5525, 2003.
  12. Tsuji, K., Yamauchi, K., Jiang, P., Yang, M., and Hoffman, R.M. Common bile duct injection as a novel method for establishing RFP-expressing human pancreatic cancer in nude mice. Journal of the Pancreas 7, 193-199, 2006.
  13. Katz, M., Spivack, D., Takimoto, S., Bouvet, M., Moossa, A.R., R.M. Hoffman. An imageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer. Clin. Exp. Metastasis 21, 7-12, 2004.
  14. Katz, M.H., Bouvet, M., Takimoto, S., Spivack, D., Moossa, A.R., and Hoffman, R.M. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer. Cancer Research 64, 1828-1833, 2004.
  15. Bouvet, M., Spernyak, J., Katz, M.H., Mazurchuk, R.V., Takimoto, S., Bernacki, R., Rustum, Y.M., Moossa, A.R., and Hoffman, R.M. High correlation of whole-body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer. Cancer Research 65, 9829-9833, 2005.
  16. Amoh, Y., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M. Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine. J. Surgical Research 132, 164-169, 2006
  17. Amoh, Y., Nagakura, C., Maitra, A., Moossa, A.R., Katsuoka, K., Hoffman, R.M., and Bouvet, M. Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.
  18. Bouvet, M., Hoffman R.M. Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X-1 - 11, 2007.
  19. McElroy, M., Kaushal, S., Tran Cao, H.S., Moossa, A.R., Talamini, M.A., Hoffman, R.M., Bouvet, M. Upregulation of thrombospondin-1 and angiogenesis in an aggressive human pancreatic cancer cell line selected for high metastasis. Molecular Cancer Therapeutics 8, 1779-1786, 2009.
  20. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M. A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007.
  21. Tran Cao, H.S., Kimura, H., Kaushal, S., Snyder, C.S., Reynoso, J., Hoffman, R.M., and Bouvet, M. The cyan fluorescent protein (CFP) transgenic mouse as a model for imaging pancreatic exocrine cells. Journal of the Pancreas 10, 152-156, 2009.
  22. Tran Cao, H.S., Reynoso, J., Yang M., Kimura, H., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet M. Development of the transgenic cyan fluorescent protein (CFP)-expressing nude mouse for “Technicolor” cancer imaging. J. Cell. Biochem 107, 328-334, 2009.
  23. Snyder, C.S., Kaushal, S., Kono, Y., Tran Cao, H.S., Hoffman, R.M., and Bouvet, M. Complementarity of ultrasound and fluorescence imaging in an orthotopic mouse model of pancreatic cancer. BMC Cancer 9, 106, 2009. DOI: 10.1117/1.3103783.
  24. Matsuo, Y., Raimondo, M., Woodward, T.A., Wallace, M.B., Gill, K.R., Tong, Z., Burdick, M.D., Yang, Z., Strieter, R.M., Hoffman, R.M., Guha, S. CXC-chemokine/CXCR2 biological axis promotes angiogenesis in vitro and in vivo in pancreatic cancer. Int. J. Cancer 125, 1027-1037, 2009.
  25. Nagakura, C., Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice. Anticancer Res. 29, 1873-1878, 2009.
  26. Zhou, J., Yu, Z., Zhao, S., Hu, L., Zheng, J., Yang, D., Bouvet, M., Hoffman, R.M. Lentivirus-based DsRed-2-transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease. J. Surg. Res. 157, 63-70, 2009.
  27. Bouvet, M., and Hoffman, R.M. Clinically-relevant orthotopic metastatic models of pancreatic cancer imageable with fluorescent genetic reporters. Minerva Chirurgica 64, 521-539, 2009.
  28. Logan-Collins, J., Thomas, R.M., Yu, P., Jaquish, D., Mose, E., French, R., Stuart, W., McClaine, R., Aronow, B., Hoffman, R.M., Waltz, S.E., and Lowy, A.M. Silencing of RON receptor signaling promotes apoptosis and gemcitabine sensitivity in pancreatic cancers. Cancer Res. 70, 1130-40, 2010. DOI: 10.1158/0008-5412.CAN-09-0761.
  29. Murphy, E.A., Shields, D.J., Stoletov, K., Dneprovskaia, E., McElroy, M., Greenberg, J.I., Lindquist, J., Acevedo, L.M., Anand, S., Majeti, B.K., Tsigelny, I., Saldanha, A., Walsh, B., Hoffman, R.M., Bouvet, M., Klemke, R.L., Vogt, P.K., Arnold, L., Wrasidlo, W., and Cheresh, D.A. Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFRb/B-RAF. Proc. Natl. Acad. Sci. USA 107, 4299-4304, 2010. DOI: 10.1073/pnas.0909299107.
  30. Tran Cao, H.S., Bouvet, M., Kaushal, S., Keleman, A., Romney, E., Kim, G., Fruehauf, J., Imagawa, D.K., Hoffman, R.M., and Katz, M. Metronomic gemcitabine in combination with Sunitinib inhibits multisite metastasis and increases survival in an orthotopic model of pancreatic cancer. Molecular Cancer Therapeutics 9, 2068-2078, 2010. (Highlights Section. Molecular Cancer Therapeutics 9, 1929, 2010).
  31. Tran Cao, H., Kaushal, S., Lee, C., Snyder, C.S., Thompson, K.J., Horgan, S., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Fluorescence laparoscopy imaging of pancreatic tumor progression in an orthotopic mouse model. Surg. Endosc. 25, 48-54, 2011.
  32. Tran Cao, H.S., Kaushal, S., Menen, R.S., Metildi, C.A., Lee, C., Snyder, C.S., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Submillimeter-resolution fluorescence laparoscopy of pancreatic cancer in a carcinomatosis mouse model visualizes metastases not seen with standard laparoscopy. J. Laparoendosc. Adv. Surg. Tech. A 21, 485-489, 2011 (Cover story).
  33. Grzesiak, J.J., Tran Cao, H.S., Burton, D.W., Kaushal, S., Vargas, F., Clopton, P., Snyder, C.S., Deftos, L.J., Hoffman, R.M., and Bouvet, M. Knockdown of the β1 integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. International Journal of Cancer 129, 2905-2915, 2011.
  34. Hassanein, M.K., Suetsugu, A., Saji, S., Moriwaki, H., Bouvet, M., Moossa, A.R., and Hoffman, R.M. Stem-like and non-stem human pancreatic cancer cells distinguished by morphology and metastatic behavior. Journal of Cellular Biochemistry 112, 3549-3554, 2011 (Cover story).
  35. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens. Anticancer Research 32, 1175-1180, 2012.
  36. Kelber, J.A., Reno, T., Kaushal, S., Metildi, C., Wright, T., Stoletov, K., Weems, J.M., Park, F.D., Mose, E., Wang, Y., Hoffman, R.M., Lowy, A.M., Bouvet, M., and Klemke, R.L. KRas induces a Src/PEAK1/ErbB2 kinase amplification loop that drives metastatic growth and therapy resistance in pancreatic cancer. Cancer Research 72, 2554-2564,2012.
  37. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Moriwaki, H., Bouvet, M., Saji, S., and Hoffman, R.M. Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens. J. Cell. Biochem. 113, 2290-2295, 2012.
  38. Metildi, C.A., Kaushal, S., Lee, C., Hardamon, C.R., Snyder, C.S., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M. An LED light source and novel fluorophore combinations improve fluorescence laparoscopic detection of metastatic pancreatic cancer in orthotopic mouse models. J. Am. Coll. Surg. 214, 997-1007, 2012.
  39. Bouvet, M., and Hoffman, R.M. In vivo imaging of pancreatic cancer with fluorescent proteins in mouse models (Chap. 4). In In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols. Hoffman, R.M., ed. Methods in Molecular Biology, Vol. 872. Walker, John M., series ed. Humana Press (Springer Science+Business Media New York), 2012.
  40. Yu, Z., Zhou, J., and Hoffman, R.M. Lentivirus-based DsRed-2-transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease (Chap. 5). In: In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols. Hoffman, R.M., ed. Methods in Molecular Biology, Vol. 872. Walker, John M., series ed. Humana Press (Springer Science+Business Media New York), 2012.
  41. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer-patient tumorgraft progression in nude mice. Anticancer Research 32, 3063-3068, 2012.
  42. Tran Cao, H.S., Kaushal, S., Metildi, C.A., Menen, R.S., Lee, C., Snyder, C.S., Messer, K., Pu, M., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Tumor-specific fluorescence antibody imaging enables accurate staging laparoscopy in an orthotopic model of pancreatic cancer. Hepato-Gastroenterology 59(118), 1994-1999, 2012. DOI: 10.5754/hge11836.
  43. Menen, R.S., Hassanein, M.K., Momiyama, M., Suetsugu, A., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Tumor-educated macrophages promote tumor growth and peritoneal metastasis in an orthotopic nude mouse model of human pancreatic cancer. In Vivo 26, 565-569, 2012.
  44. Menen, R.S., Hassanein, M.K., Momiyama, M., Suetsugu, A., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Tumor-educated macrophages promote tumor growth and peritoneal metastasis in an orthotopic nude mouse model of human pancreatic cancer. In Vivo 26, 565-569, 2012.
  45. Shi, Y., Tong, M., Wu, Y., Yang, Z., Hoffman, R.M., Zhang, Y., Tian, Y., Qi, M., Lin, Y., Liu, Y., Dai, L., Sun, Y., and Wang, Z. VEGF-C ShRNA inhibits pancreatic cancer growth and lymphangiogenesis in an orthotopic fluorescent nude mouse model. Anticancer Research 33, 409-417, 2013.
  46. Snyder, C.S., Harrington, A.R., Kaushal, S., Mose, E., Lowy, A.M., Hoffman, R.M., and Bouvet, M. A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment. Pancreas 42, 952-958, 2013.
  47. Zhang, L., Wu, C., Zhang, Y., Liu, F., Zhao, M., Bouvet, M., and Hoffman, R.M. Efficacy comparison of traditional Chinese medicine LQ versus gemcitabine in a mouse model of pancreatic cancer. J. Cell. Biochem. 114, 2131-2137, 2013.
  48. Hiroshima, Y., Zhao, M., Zhang, Y., Maawy, A., Hassanein, M.K., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Suetsugu, A., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. Cell Cycle 12, 2774-2780, 2013.
  49. Miwa, S., Yano, S., Hiroshima, Y., Tome, Y., Uehara, F., Mii, S., Efimove, E.V., Kimura, H., Hayashi, K., Tsuchiya, H., Hoffman, R.M. Imaging UVC-induced DNA damage response in models of minimal cancer. J. Cell. Biochem. 114, 2493-2499, 2013.
  50. Metildi, C.A., Kaushal, S., Hoffman, R.M., and Bouvet, M. In vivo serial selection of human pancreatic cancer cells in orthotopic mouse models produces high metastatic variants irrespective of Kras status. J. Surg. Res. 184, 290-298, 2013.
  51. Maawy, A.A., Hiroshima, Y., Kaushal, S., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models. J. Biomed. Optics 18, 126016, 2013.
  52. Metildi, C.A., Kaushal, S., Pu, M., Messer, K.A., Luiken, G.A., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Fluorescence-guided surgery with a fluorophore-conjugated antibody to carcinoembryonic antigen (CEA), that highlights the tumor, improves surgical resection and increases survival in orthotopic mouse models of human pancreatic cancer. Ann. Surg. Oncol. 21, 1405-1411, 2014.
  53. Hiroshima, Y., Maawy, A., Sato, S., Murakami, T., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice. J. Surg. Res. 187, 510-517, 2014.
  54. Hiroshima, Y., Zhao, M., Maawy, A., Zhang, Y., Katz, M.H., Fleming, J.B., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Suetsugu, A., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Efficacy of Salmonella typhimurium A1-R versus chemotherapy on a pancreatic cancer patient-derived orthotopic xenograft (PDOX). J. Cell. Biochem. 115, 1254-1261, 2014.
  55. Metildi, C.A., Kaushal, S., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Advantages of fluorescence-guided laparoscopic surgery of pancreatic cancer labeled with fluorescent anti-carcinoembryonic antigen antibodies in an orthotopic mouse model. J Am. Coll. Surg. 219, 132-141, 2014.
  56. Hiroshima, Y., Maawy, A., Zhang, Y., Sato, S., Murakami, T., Yamamoto, M., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Fluorescence-guided surgery in combination with UVC irradiation cures metastatic human pancreatic cancer in orthotopic mouse models. PLoS One 9, e99977, 2014.
  57. Maawy, A., Hiroshima, Y., Zhang, Y., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Specific tumor labeling enhanced by polyethylene glycol linkage of near infrared dyes conjugated to a chimeric anti-carcinoembryonic antigen antibody in a nude mouse model of human pancreatic cancer. J. Biomed. Optics 19, 101504, 2014.
  58. Hiroshima, Y., Maawy, A., Hassanein, M.K., Menen, R., Momiyama, M., Murakami, T., Miwa, S., Yamamoto, M., Uehara, F., Yano, S., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M.. The tumor-educated-macrophage increase of malignancy of human pancreatic cancer is prevented by Zoledronic acid. PLoS One 9(8), e103382, 2014.
  59. Metildi, C.A., Kaushal, S., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Advantages of fluorescence-guided laparoscopic surgery of pancreatic cancer labeled with fluorescent anti-carcinoembryonic antigen antibodies in an orthotopic mouse model. J Am. Coll. Surg. 219, 132-141, 2014.
  60. Uehara, F., Miwa, S., Tome, Y., Hiroshima, Y., Yano, S., Yamamoto, M., Efimova, E., Matsumoto, Y., Maehara, H., Bouvet, M., Kanaya, F., and Hoffman, R.M. Comparison of UVB and UVC effects on the DNA damage-response protein 53BP1 in human pancreatic cancer. J. Cell. Biochem. 115, 1724-1728, 2014.
  61. Yao, W-T., Wu, J-F., Yu, G-Y., Wang, R., Wang, K., Li, L-H., Chen, P., Jiang, Y-N., Cheng, H., Lee, H.W., Yu, J., Qi, H., Yu, X-J., Wang, P., Chu, Y-W., Yang, M., Hua, Z-C., Ying, H-Q., Hoffman, R.M., Jeong, L.S., Jia, L-J. Suppression of tumor angiogenesis by targeting the protein neddylation pathway. Cell Death & Disease 5, e1059, 2014.
  62. Hiroshima, Y., Maawy, A., Zhang, Y., Murakami, T., Momiyama, M., Mori, R., Matsuyama, R., Katz, M.H., Fleming, J.B., Chishima, T., Tanaka, K., Ichikawa, Y., Endo, I., Hoffman, R.M., and Bouvet, M. Metastatic recurrence in a pancreatic cancer patient derived orthotopic xenograft (PDOX) nude mouse model is inhibited by neoadjuvant chemotherapy in combination with fluorescence-guided surgery with an anti-CA 19-9-conjugated fluorophore. PLoS One 9, e114310, 2014.
  63. Hiroshima, Y., Zhang, Y., Murakami, T., Maawy, A.A., Miwa, S., Yamamoto, M., Yano, S., Sato, S., Momiyama, M., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Endo, I., Zhao, M., and Hoffman, R.M. Efficacy of tumor-targeting Salmonella typhimurium A1-R in combination with anti-angiogenesis therapy on a pancreatic cancer patient-derived orthotopic xenograph (PDOX) and cell line mouse models. Oncotarget 5, 12346-12357, 2015.
  64. Bouvet, M., and Hoffman, R.M. Fluorescence-guided surgery: It is the cure that matters. In reply, J. Am. Coll. Surg. 220, 377-379, 2015.
  65. Park, J.Y., Hiroshima, Y., Lee, J.Y., Maawy, A.A., Hoffman, R.M., and Bouvet, M. MUC1 selectively targets human pancreatic cancer in orthotopic nude mouse models. PLoS One, 10, e0122100, 2015.
  66. Maawy, A.A., Hiroshima, Y., Zhang, Y., Heim, R., Makings, L., Garcia-Guzman, M., Luiken, G.A., Kobayashi, H., Hoffman, R.M., and Bouvet, M. Near infra-red photoimmunotherapy with anti-CEA-IR700 results in extensive tumor lysis and a significant decrease in tumor burden in orthotopic mouse models of pancreatic cancer. PLoS One 10, e0121989, 2015.
  67. Suetsugu, A., Snyder, C.S., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M. Imaging the interaction of pancreatic cancer and stellate cells in the tumor microenvironment during metastasis. Anticancer Res. 35, 2545-2551, 2015.
  68. Hiroshima, Y., Maawy, A., Zhan, Y., Murakami, T., Momiyama, M., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Endo, I., Hoffman, R.M., and Bouvet, M. Fluorescence-guided surgery, but not bright-light surgery, prevents local recurrence in a pancreatic cancer patient-derived orthotopic xenograft (PDOX) model resistant to neoadjuvant chemotherapy (NAC). Pancreatology 15, 295-301, 2015.
  69. Metildi, C.A., Felsen, C.N., Savariar, E.N., Nguyen, Q.T., Kaushal, S., Hoffman, R.M., Tsien, R.Y., and Bouvet, M. Ratiometric activatable cell-penetrating peptides label pancreatic cancer, enabling fluorescence-guided surgery, which reduces metastases and recurrence in orthotopic mouse models. Ann. Surg. Oncol. 22, 2082-2087, 2015.
  70. Maawy, A.A., Hiroshima, Y., Zhang, Y., Garcia-Guzman, M., Luiken, G.A., Kobayashi, H., Hoffman, R.M., and Bouvet, M. Photoimmunotherapy lowers recurrence after pancreatic cancer surgery. J. Surg. Res. 197, 5-11, 2015.
  71. Yano, S., Hiroshima, Y., Maawy, A., Kishimoto, H., Suetsugu, A., Miwa, S., Toneri, M., Yamamoto, M., Katz, M.H.G., Fleming, J.B., Urata, Y., Tazawa, H., Kagawa, S., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery. Cancer Gene Therapy 22, 344-350, 2015.
  72. Hoffman, R.M., Bouvet, M. Nab-paclitaxel: a limited improvement under the current therapeutic paradigm of pancreatic cancer. Expt Op Pharmacotherapy 16, 943-947, 2015.
  73. Jun, E., Jung, J., Jeong, S-Y., Choi, E.K., Kim, M. B., Lee, J. S., Hong, S-M., Seol, H. S., Hwang, C., Hoffman, R.M., Shim, I. K., Chang, S., and Kim, S. C. Surgical and oncological factors affecting the successful engraftment of patient-derived xenografts in pancreatic ductal adenocarcinoma. Anticancer Res. 36, 517-522, 2016.
  74. 73. Bouvet, M., and Hoffman, R.M. Toward curative fluorescence-guided surgery of pancreatic cancer. Hepato-Gastroenterology 62, 715-722, 2015. DOI: 10.5754/hge15001
  75. Hoffman, R.M., and Bouvet, M. Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP. Cancer Letters (Special Issue: Tumor Microenvironment. Singh, Shree Ram, ed.), 380, 349-355, 2016.
  76. Hoover, M., Adamian, Y., Brown, M., Maawy, A., Chang, A., Lee, J., Gharibi, A., Katz, M.H., Fleming, J., Hoffman, R.M., Bouvet, M., Doebler, R., and Kelber J.A. A novel method for RNA extraction from FFPE samples reveals significant differences in biomarker expression between orthotopic and subcutaneous pancreatic cancer patient-derived xenografts. Oncotarget 8, 5885-5894, 2017.

Prostate Cancer & Metastasis

  1. Miwa, S., Matsumoto, Y., Hiroshima, Y., Yano, S., Uehara, F., Yamamoto, M., Zhang, Y., Kimura, H., Hayashi, K., Yamamoto, N., Bouvet, M., Sugimoto, N., Tsuchiya, H., Hoffman, R.M. Fluorescence-guided surgery of prostate cancer bone metastasis. J. Surgical Research 192, 124-133, 2014.

Prostate

  1. Fu, X., Herrera, H., and Hoffman, R.M. Orthotopic growth and metastasis of human prostate carcinoma in nude mice after transplantation of histologically intact tissue. Int. J. Cancer 52, 987-990, 1992.
  2. An, Z., Wang, X., Geller, J., Moossa, A.R., and Hoffman, R.M. Surgical orthotopic implantation allows high lung and lymph node metastatic expression of human prostate carcinoma cell line PC-3 in nude mice. The Prostate 34, 169-174, 1998.
  3. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Res. 59, 781-786, 1999.
  4. Wang, X., An, Z., Geller, J., and Hoffman, R.M. High malignancy orthotopic nude mouse model of human prostate cancer LNCaP. The Prostate 39, 182-186, 1999.
  5. Glinskii, A.B., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M., Glinsky, G.V. Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models. Cancer Res. 63, 4239-4243, 2003.
  6. Glinsky, G.V., Glinskii, A.B., Stephenson, A.J., Hoffman, R.M., and Gerald, W.L. Gene expression profiling predicts clinical outcome of prostate cancer. J. Clin. Investig. 113, 913-923, 2004.
  7. Yang, M., Jiang, P., Yamamoto, N., Li, L., Geller, J., Moossa, A.R., and Hoffman, R.M. Real-time whole-body imaging of an orthotopic metastatic prostate cancer model expressing red fluorescent protein. The Prostate 62, 374-379, 2005.
  8. Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005.
  9. Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005.
  10. Berezovskaya, O., Schimmer, A.D., Glinskii, A.B., Pinilla, C., Hoffman, R.M., Reed, J.C., and Glinsky, G.V. Increased expression of apoptosis inhibitor protein XIAP contributes to anoikis resistance of circulating human prostate cancer metastasis precursor cells. Cancer Research 65, 2378-2386, 2005.
  11. Glinsky, G.V., Glinskii, A.B., Berezovskaya, O., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M. Dual-color-coded imaging of viable circulating prostate carcinoma cells reveals genetic exchange between tumor cells in vivo, contributing to highly metastatic phenotypes. Cell Cycle 5, 191-197, 2006.
  12. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model. Clinical Cancer Research 12, 2602-2606, 2006.
  13. Berezovska, O.P., Glinskii, A.B., Yang, Z., Li, X.M., Hoffman, R.M., and Glinsky, G.V. Essential role for activation of the Polycomb Group (PcG) protein chromatin silencing pathway in metastatic prostate cancer. Cell Cycle 5, 1886-1901, 2006.
  14. Hoffman, R.M. Orthotopic metastatic mouse models of prostate cancer. In: Metastasis of Prostate Cancer, pp. 143-169. Ablin, R.J. and Mason, M.D., eds. Kluwer Academic Publishers, 2007.
  15. Zhang, Q., Yang, M., Shen, J., Gerhold, L.M., Hoffman, R.M., Xing, H.R. The role of the intravascular microenvironment in spontaneous metastasis development. International Journal of Cancer 126, 2534-2541, 2010.
  16. Kolostova, K., Pinterova, D., Hoffman, R.M., and Bobek, V. Circulating human prostate cancer cells from an orthotopic mouse model rapidly captured by immunomagnetic beads and imaged by GFP expression. Anticancer Res. 31, 1535-1539, 2011.
  17. Menen, R.S., Pinney, E., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M. A rapid imageable in vivo metastasis assay for circulating tumor cells. Anticancer Res. 31, 3125-3128, 2011.
  18. Menen, R., Pinney, E., Hassanein, M.K., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M. Inhibition of metastasis of circulating human prostate cancer cells in the chick embryo by an extracellular matrix produced by foreskin fibroblasts in culture. Anticancer Research 32, 1573-1577, 2012.
  19. Menen, R., Zhao, M., Zhang, L., Hassanein, M.K., Bobek, V., Kolostova, K., Bouvet, M., and Hoffman, R.M. Comparative chemosensitivity of circulating human prostate cancer cells and primary cancer cells. Anticancer Research 32, 2881-2884, 2012.
  20. Ongkeko, W.M., Burton, D., Kiang, A., Abhold, E., Kuo, S.Z., Rahimy, E., Yang, M., Hoffman, R.M., Wang-Rodriguez, J., Deftos, L.J. Parathyroid hormone related-protein promotes epithelial-to-mesenchymal transition in prostate cancer. PLoS One 9, e85803, 2014.
  21. Miwa, S., Matsumoto, Y., Hiroshima, Y., Yano, S., Uehara, F., Yamamoto, M., Zhang, Y., Kimura, H., Hayashi, K., Yamamoto, N., Bouvet, M., Sugimoto, N., Tsuchiya, H., Hoffman, R.M. Fluorescence-guided surgery of prostate cancer bone metastasis. J. Surgical Research 192, 124-133, 2014.
  22. Toneri, M., Miwa, S., Zhang, Y., Hu, C., Yano, S., Matsumoto, Y., Bouvet, M., Nakanishi, H., Hoffman, R.M., and Zhao, M. Tumor-targeting Salmonella typhimurium A1-R inhibits human prostate cancer experimental bone metastasis in mouse models. Oncotarget 6, 31335-31343, 2015.
  23. Zhang, L., Wu, C., and Hoffman, R.M. Prostate cancer heterogeneous high-metastatic multi-organ-colonizing chemo-resistant variants selected by serial metastatic passage in nude mice are highly enriched for multinucleate giant cells. PLoS One 10, e0140721, 2015.
  24. Zhang, Y., Wang, X., Hoffman, R.M., and Seki, N. Real time metastatic route tracking of orthotopic PC-3-GFP human prostate cancer using intravital imaging. J. Cell. Biochem. 117, 1027-1032, 2016.
  25. Zhang, Y., Toneri, M., Ma, H., Yang, Z., Bouvet, M., Goto, Y., Seki, N., and Hoffman, R.M. Real-time GFP intravital imaging of the difference in cellular and angiogenic behavior of subcutaneous and orthotopic nude-mouse models of human PC-3 prostate cancer. J. Cellular Biochemistry 117, 2546-2551, 2016.
  26. Miwa, S., Toneri, A., Igarashi, K., Yano, S., Kimura, H., Hayashi, K., Yamamoto, N., Tsuchiya, H., Hoffman, R.M. Real-time in vivo confocal fluorescence imaging of prostate cancer bone-marrow micrometastasis development at the cellular level in nude mice. J. Cell. Biochem. 117, 2533-2537, 2016.

Rectal

  1. Kishimoto, H., Momiyama, M., Aki, R., Kimura, H., Suetsugu, A., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Development of a clinically-precise mouse model of rectal cancer. PLoS One 11, e79453, 2013.

Sarcoma

  1. Yamamoto, N., Yang, M., Jiang, P., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases. Clin. Exp. Metastasis 20, 181-185, 2003.
  2. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time imaging of individual fluorescent protein color-coded metastatic colonies in vivo. Clin. Exp. Metastasis 20(7), 633-638, 2003.
  3. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging. Cancer Research 63, 7785-7790, 2003.
  4. Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G.M., Moossa, A.R., and Hoffman, R.M. Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Research 64, 4251-4256, 2004.
  5. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies. Anticancer Research 24, 4067-4072, 2004.
  6. Yamauchi, K., Yang, M., Jiang, P., Yamamoto, N., Xu, M., Amoh, Y., Tsuji, K., Bouvet, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time in vivo dual-color imaging of intracapillary cancer cell and nucleus deformation and migration. Cancer Res. 65, 4246-4252, 2005.
  7. Yamauchi, K., Yang, M., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system. Cancer Res. 66, 4208-4214, 2006.
  8. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M. Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007.
  9. Hayashi, K., Jiang, P., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res. 67, 8223-8228, 2007.
  10. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Imaging of nucleolar dynamics during the cell cycle of cancer cells in live mice. Cell Cycle 6, 2706-2708, 2007.
  11. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008.
  12. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Imaging enhancement of malignancy by cyclophosphamide: surprising chemotherapy opposite effects. Proc. of SPIE 6868. 68680I1-68680I5, 2008.
  13. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. Cell Cycle 8, 870-875, 2009.
  14. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., and Hoffman, R.M. Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhymurium. J. Cell. Biochem. 106, 992-998, 2009.
  15. Kimura, H., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Real-time imaging of single cancer-cell dynamics of lung metastasis. J. Cell. Biochem. 109, 58-64, 2010.
  16. Kimura, H., Lee, C., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo. J. Cell. Biochem 110, 1439-1446, 2010 (Cover Story).
  17. Kimura, H., Momiyama, M., Tomita, K., Tsuchiya, H., and Hoffman, R.M. Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular. J. Biomed. Optics 15(6), 066029, 2010.
  18. Yamamoto, N., Tsuchiya, H., and Hoffman, R.M. Tumor imaging with multicolor fluorescent protein expression. Int. J. Clin. Oncol. 16, 84-91, 2011
  19. Hayashi, K., Kimura, H., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Hasegawa, A., Bouvet, M., and Hoffman, R.M. Comparison of cancer cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse. Anticancer Res. 31, 3665-3672, 2011.
  20. Yamauchi, K., Tome, Y., Yamamoto, N., Hayashi, K., Kimura, H., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman R.M. Color-coded real-time subcellular fluorescence imaging of the interaction between cancer and host cells in live mice. Anticancer Res. 32, 39-43, 2012.
  21. Kimura, H., Tome, Y., Momiyama, M., Hayashi, K., Tsuchiya, H., Bouvet, M., and Hoffman, R.M. Imaging the inhibition by anti-b1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int. J. Cancer 131, 2027-2033, 2012. DOI: 10.1002/ijc.27475.
  22. Uehara, F., Tome, Y., Miwa, S., Hiroshima, Y., Yano, S., Yamamoto, M., Mii, S., Maehara, H., Bouvet, M., Kanaya, F., and Hoffman, R.M. Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam® assay. J. Cell. Biochem. 115, 1490-1494, 2014.
  23. Golubkov, V.S., Prigozhina, N.L., Zhang, Y., Stoletov, K., Lewis, J.D., Schwartz, P.E., Hoffman, R.M., and Strongin, A.Y. Protein-tyrosine pseudokinase 7 (PTK7) directs cancer cell motility and metastasis. J. Biol. Chem. 289, 24238-24249, 2014.
  24. Tome, Y., Tsuchiya, H., Hayashi, K., Yamauchi, K., Sugimoto, N., Kanaya, F., Tomita, K., and Hoffman, R.M. In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. J. Cell. Biochem. 108, 362- 367, 2009. DOI: 10.1002/jcb.22259.
  25. Uehara, F., Tome, Y., Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Bouvet, M., Maehara, H., Kanaya, F., and Hoffman, R.M. A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res. 33, 1361-1366, 2013.
  26. Tome, Y., Sugimoto, N., Yano, S., Momiyama, M., Mii, S., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Research 33, 3021-3025, 2013.
  27. Tome, Y., Kimura, H., Maehara, H., Sugimoto, N., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of αvβ3 integrin. Anticancer Research 33, 3623-3627, 2013.
  28. Uehara, F., Tome, Y., Miwa, S., Hiroshima, Y., Yano, S., Yamamoto, M., Mii, S., Maehara, H., Bouvet, M., Kanaya, F., and Hoffman, R.M. Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam® assay. J. Cell. Biochem. 115, 1490-1494, 2014.
  29. Miwa, S., Hiroshima, Y., Yano, S., Zhang, Y., Matsumoto, Y., Uehara, F., Yamamoto, M., Kimura, H., Hayashi, K., Bouvet, M., Tsuchiya, H., and Hoffman R.M. Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model. J Orthopaedic Res. 32, 1596-1601, 2014.
  30. Miwa, S., Zhang, Y., Baek, K-E., Uehara, F., Yano, S., Yamamoto, M., Hiroshima, Y., Matsumoto, Y., Kimura, H., Hayashi, K., Yamamoto, N., Tsuchiya, H., Hoffman, R.M., and Zhao, M. Inhibition of spontaneous and experimental lung metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R. Oncotarget 5, 12849-12861, 2014.
  31. Yano, S., Miwa, S., Kishimoto, H., Uehara, F., Tazawa, H., Toneri, M., Hiroshima, Y., Yamamoto, M., Urata, Y., Kagawa, S., Bouvet, M., Funiwara, T., and Hoffman, R.M. Targeting tumors with a killer-reporter adenovirus for curative fluorescence-guided surgery of soft-tissue sarcoma. Oncotarget 6, 13133-13148, 2015.
  32. Hiroshima, Y., Zhao, M., Zhang, Y., Zhang, N., Maawy, A., Murakami, T., Mii, S., Uehara, F., Yamamoto, M., Miwa, S., Yano, S., Momiyama, M., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R arrests a chemo-resistant patient soft-tissue sarcoma in nude mice. PLoS One 10, e0134324, 2015.
  33. Murakami, T., DeLong, J., Eilber, F.C., Zhao, M., Zhang, Y., Zhang, N., Singh, A., Russell, T., Deng, S., Reynoso, J., Quan, C., Hiroshima, Y., Matsuyama, R., Chishima, T., Tanaka, K., Bouvet, M., Chawla, S., Endo, I., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R in combination with doxorubicin eradicate soft tissue sarcoma in a patient-derived orthotopic xenograft PDOX model. Oncotarget 7, 12783-12790, 2016.
  34. Kiyuna, T., Murakami, T., Tome, Y., Kawaguchi, K., Igarashi, K., Zhang, Y, Zhao, M., Li, Y., Bouvet, M., Kanaya, F., Singh, A., Dry, S., Eilber, F.C., and Hoffman, R.M. High efficacy of tumor-targeting Salmonella typhimurium A1-R on a doxorubicin- and dactolisib-resistant follicular dendritic-cell sarcoma in a patient-derived orthotopic xenograft PDOX nude mouse model. Oncotarget 7, 33046-33054, 2016.
  35. Binder, D.C., Arina, A., Wen, F., Tu, T., Zhao, M., Hoffman, R.M., Wainwright, D.A., and Schreiber, H. Tumor relapse prevented by combining adoptive T cell therapy with Salmonella typhimurium. Oncoimmunology 5, e1130207, 2016. DOI: 10.1080/2162402X.2015.1130207.
  36. Tome, Y., Yano, S., Sugimoto, N., Mii, S., Uehara, F., Miwa, S., Bouvet, M., Tsuchiya, H., Kanaya, F., Hoffman, R.M. Use of αv integrin linked to green fluorescent protein in osteosarcoma cells and confocal microscopy to image molecular dynamics during lung metastasis in nude mice. Anticancer Research 36, 3811-3816, 2016.
  37. Murakami, T., Singh, A.S., Kiyuna, T., Dry, S.M., Li, Y., James, A.W., Igarashi, K., Kawaguchi, K., DeLong, J.C., Zhang, Y., Hiroshima, Y., Russell, T., Eckardt, M.A., Yanagawa, J., Federman, N., Matsuyama, R., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., Eilber, F.C., and Hoffman, R.M. Effective molecular targeting of CDK4/6 and IGF-1R in a rare FUS-ERG fusion CDKN2A-deletion doxorubicin-resistant Ewing’s sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model. Oncotarget 7, 47556-47564, 2016.
  38. Kiyuna, T., Murakami, T., Tome, Y., Igarashi, K., Kawaguchi, K., Russell, T., Eckhardt, M.A., Crompton, J., Singh, A., Bernthal, N., Bukata, S., Federman, N., Kanaya, F., Eilber, F.C., and Hoffman, R.M. Labeling the stroma of a patient-derived orthotopic xenograft (PDOX) mouse models of undifferentiated pleomorphic soft-tissue sarcoma with red fluorescent protein for rapid non-invasive drug screening. J. Cell. Biochem. 118, 361-365, 2017.
  39. Igarashi, K., Kawaguchi, K., Kiyuna, T., Murakami, T., Miwa, S., Nelson, S.D., Dry, S.M., Li, Y., Singh, A., Kimura, H., Hayashi, K., Yamamoto, N., Tsuchiya, H., Eilber, F.C., Hoffman, R.M. Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model. Cell Cycle 16, 91-94, 2017.
  40. Murakami, T., Igarashi, K., Kawaguchi, K., Kiyuna, T., Zhang, Y., Zhao, M., Hiroshima, Y., Nelson, S.D., Dry, S.M., Li, Y., Yanagawa, J., Russell, T., Federman, N., Singh, A., Elliott, I., Matsuyama, R., Chishima, T., Tanaka, K., Endo, I., Eilber, F.C., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R regresses an osteosarcoma in a patient-derived xenograph model resistant to a molecular-targeting drug. Oncotarget 8, 8035-8042, 2017.
  41. Tome, Y., Yano, S., Sugimoto, N., Mii, S., Bouvet, M., Tsuchiya, H., Kanaya, F., Hoffman, R.M. Use of αv integrin linked to GFP to image molecular dynamics in trafficking cancer-cell emboli. J. Cell. Biochem. 118, 26-30, 2017. (Cover story)
  42. Kiyuna, T., Murakami, T., Tome, Y., Igarashi, K., Kawaguchi, K., Russell, T., Eckhardt, M.A., Crompton, J., Singh, A., Bernthal, N., Bukata, S., Federman, N., Kanaya, F., Eilber, F.C., and Hoffman, R.M. Labeling the stroma of a patient-derived orthotopic xenograft (PDOX) mouse models of undifferentiated pleomorphic soft-tissue sarcoma with red fluorescent protein for rapid non-invasive drug screening. J. Cell. Biochem. 118, 361-365, 2017.
  43. Tome, Y., Kimura, H., Sugimoto, N., Tsuchiya, H., Kanaya, F., Bouvet, M., Hoffman, R.M. The disintegrin echistatin, in combination with doxorubicin targets high-metastastic human osteosarcoma overexpressing ανβ3 integrin in chick embryo and nude mouse models. Oncotarget 7, 87031-87036, 2016.

Stomach

  1. Furukawa, T., Fu, X., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M. Nude mouse metastatic models of human stomach cancer constructed using orthotopic implantation of histologically intact tissue. Cancer Res. 53, 1204-1208, 1993.
  2. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M. Orthotopic transplantation of histologically intact clinical specimens of stomach cancer to nude mice: Correlation of metastatic sites in mouse and individual patient donors. Int. J. Cancer 53, 608-612, 1993.
  3. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Kitajima, M., and Hoffman, R.M. Differential chemosensitivity of local and metastatic human gastric cancer after orthotopic transplantation of histologically intact tumor tissue in nude mice. Int. J. Cancer 54, 397-401, 1993.
  4. Inada, T., Ichikawa, A., Kubota, T., Ogata, Y., Moossa, A.R., and Hoffman, R.M. 5-FU-induced apoptosis correlates with efficacy against human gastric and colon cancer xenografts in nude mice. Anticancer Res. 17, 1965-1971, 1997.
  5. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.
  6. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H. VEGF receptor antisense therapy inhibits angiogenesis and peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy 9, 197-201, 2002.
  7. Yano, S., Zhang, Y., Miwa, S., Tome, Y., Hiroshima, Y., Uehara, F., Yamamoto, M., Suetsugu, A., Kishimoto, H., Tazawa, H., Zhao, M., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Spatial-temporal FUCCI imaging of each cell in a tumor demonstrates locational dependence of cell cycle dynamics and chemoresponsiveness. Cell Cycle 13, 2110-2119, 2014.
  8. Yano, S., Zhang, Y., Zhao, M., Hiroshima, Y., Miwa, S., Uehara, F., Kishimoto, H., Tazawa, H., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R decoys quiescent cancer cells to cycle as visualized by FUCCI imaging and become sensitive to chemotherapy. Cell Cycle 13, 3958-3963, 2014.
  9. Murakami, T., Zhang, Y., Wang, X., Hiroshima, Y., Kasashima, H., Yashiro, M., Hirakawa, K., Miwa, A., Kiyuna, T., Matsuyama, R., Tanaka, K., Bouvet, M., Endo, I., Hoffman, R.M. Orthotopic implantation of tumor tissue leads to metastasis of OCUM-2MD3 human stomach cancer visualized in real time by intravital fluorescence imaging in nude mice. Anticancer Research 36, 2125-2130, 2016.

TCM

  1. Wang, M., Zhang, X., Xiong, X., Yang, Z., Sun, Y., Yang, Z., Hoffman, R.M., and Liu, Y. Efficacy of the Chinese Traditional Medicinal Herb Celastrus orbiculatus Thunb on human hepatocellular carcinoma in an orthotopic fluorescent nude mouse model. Anticancer Res. 32, 1213-1220, 2012.
  2. Hu, M., Zhao, M., An, C., Yang, M., Zhang, Y., Suetsugu, A., Tome, Y., Yano, S., Fu, Y., Hoffman, R.M., and Hu, K. Real-time imaging of induction of apoptosis of human breast cancer cells by the traditional Chinese medicine herb Tubeimu. Anticancer Res. 32, 2509-2514, 2012.
  3. Zhang, L., Wu, C., Zhang, Y., Liu, F., Zhao, M., Bouvet, M., and Hoffman, R.M. Efficacy comparison of traditional Chinese medicine LQ versus gemcitabine in a mouse model of pancreatic cancer. J. Cell. Biochem. 114, 2131-2137, 2013.
  4. Zhang, L., Wu, C., Zhang, Y., Liu, F., Wang, X., Zhao, M., and Hoffman, R.M. Comparison of efficacy and toxicity of Traditional Chinese Medicine (TCM) herbal mixture LQ and conventional chemotherapy on lung cancer metastasis and survival in mouse models. PLoS One 9, e109814, 2014.
  5. Zhang, L., Wu, C., Bouvet, M., Yano, S., and Hoffman, R.M. Traditional Chinese medicine herbal mixture LQ arrests FUCCI-expressing HeLa cells in G0/G1 phase in 2D plastic, 2.5D Matrigel®, and 3D Gelfoam® culture visualized with FUCCI imaging. Oncotarget 6, 5292-5298, 2015.
  6. Dai, X., Liu, D., Liu, M., Zhang, X., Wang, W., Jin, F., Qian, Y., Wang, X., Zhao, J., Wu, Y., Xiong, F., Chang, N., Sun Y., Yang, Z., Hoffman, R.M., Liu, Y. Anti-metastatic efficacy of traditional Chinese medicine (TCM) ginsenoside conjugated to an VEFGR-3 antibody on human gastric cancer in an orthotopic mouse model. Anticancer Res. 37, 979-986, 2017.

Thyroid

  1. Johnson, T.E., Luiken, G.A., Quigley, M.M., Xu, M., and Hoffman, R.M. In vivo fluorescence of medullary carcinoma of the thyroid: A technology with potential to improve visualization of malignant tissue at surgical resection. Ear Nose Throat Journal 87(8), E1, 2008. (Total unique citations: 2) (ISI: 1) (Google: 2) (Scopus: 1)
  2. Tran Cao, H.S., Kaushal, S., Snyder, C.S., Ongkeko, W.M., Hoffman, R.M., and Bouvet, M. Real-time imaging of tumor progression in a fluorescent orthotopic mouse model of thyroid cancer. Anticancer Research 30, 4415-4422, 2010.

Drug Discovery

  1. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T.H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M. Immunochemotherapy prevents human colon cancer metastasis after orthotopic onplantation of histologically-intact tumor tissue in nude mice. Anticancer Res. 13, 287-291, 1993.
  2. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Kitajima, M., and Hoffman, R.M. Differential chemosensitivity of local and metastatic human gastric cancer after orthotopic transplantation of histologically intact tumor tissue in nude mice. Int. J. Cancer 54, 397-401, 1993.
  3. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M. A novel "patient-like" treatment model of human pancreatic cancer constructed using orthotopic transplantation of histologically intact human tumor tissue in nude mice. Cancer Res. 53, 3070-3072, 1993.
  4. Kubota, T., Inoue, S., Furukawa, T., Ishibiki, K., Kitajima, M., Kawamura, E., and Hoffman, R.M. Similarity of serum – Tumor pharmacokinetics of antitumor agents in man and nude mice. Anticancer Res. 13, 1481-1484, 1993.
  5. Wang, X., Fu, X., Brown, P.D., Crimmin, M.J., and Hoffman, R.M. Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice. Cancer Res. 54, 4726-4728, 1994.
  6. Murray, G., Duncan, M., O’Neil, P., Melvin, W., Fothergill, J. Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer. Nature Medicine 2, 461-2, 1996.
  7. An, Z., Wang, X., Astoul, P., Danays, T., Moossa, A.R., and Hoffman, R.M. Interferon gamma is highly effective against orthotopically-implanted human pleural adenocarcinoma in nude mice. Anticancer Res. 16, 2546-2551, 1996.
  8. Olbina, G., Cieslak, D., Ruzdijic, S., Esler, C., An, Z., Wang, X., Hoffman, R., Seifert, W., and Pietrzkowski, Z. Reversible inhibition of IL-8 receptor B mRNA expression and proliferation in non-small cell lung cancer by antisense oligonucleotides. Anticancer Res. 16, 3525-3530, 1996.
  9. An, Z., Wang, X., Willmott, N., Chander, S.K., Tickle, S., Docherty, A.J., Mountain, A., Millican, A.T., Morphy, R., Porter, J.R., Epemolu, R.O., Kubota, T., Moossa, A.R., and Hoffman, R.M. Conversion of highly malignant colon cancer from an aggressive to a controlled disease by oral administration of a metalloproteinase inhibitor. Clin & Exp Metastasis 15, 184-195, 1997.
  10. Inada, T., Ichikawa, A., Kubota, T., Ogata, Y., Moossa, A.R., and Hoffman, R.M. 5-FU-induced apoptosis correlates with efficacy against human gastric and colon cancer xenografts in nude mice. Anticancer Res. 17, 1965-1971, 1997.
  11. Tomikawa, M., Kubota, T., Matsuzaki, S.W., Takahasi, S., Kitajima, M., Moossa, A.R., and Hoffman, R.M. Mitomycin C and cisplatin increase survival in a human pancreatic cancer metastatic model. Anticancer Res. 17, 3623-3626, 1997.
  12. Chang, SG., Kim, J.I., Jung, JC., Rho, YS., Lee, KT., An, Z., Wang, X., Hoffman, R.M. Antimetastatic activity of the new plati-num analog {Pt(cis-dach)(DPPE)×2NO3} in a metastatic model of human bladder cancer. Anticancer Res. 17, 3239-3242, 1997.
  13. Sasson, A.R., Gamagami, R., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M. Cimetidine: an inhibitor or promoter of tumor growth? International Journal of Cancer 81, 835-838, 1999.
  14. Rho, Y-S., Lee, K-T., Jung, J-C, Yoon, C., An, Z., Hoffman, R.M., and Chang, S-G. Efficacy of new platinum analog DPPE in an orthotopic nude mouse model of human colon cancer. Anticancer Res. 19, 157-161, 1999.
  15. Woessner, R., An, Z., Li, X., Hoffman, R.M., Dix, R., Bitonti, A. Comparison of three approaches to doxorubicin therapy: free doxorubicin, liposomal doxorubicin, and b-glucuronidase-activated prodrug (HMR 1826). Anticancer Res. 20, 2289-2296, 2000.
  16. Rashidi, B., An, Z., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Antimetastatic intraoperative chemotherapy of human colon tumors in the livers of nude mice. Clinical Cancer Res. 6, 2464-2468, 2000.
  17. Rashidi, B., An, Z., Sun, F-X, Li, X-M., Tang, Z.Y., Moossa, A.R., and Hoffman, R.M. Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice. Int. J. Cancer 91, 231-235, 2001.
  18. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., Hoffman, R.M. Anti-metastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clin & Exp Metastasis 18, 379-384, 2001.
  19. Ireson, C.R., Chander, S.K., Purohit, A., Perera, S., Newman, S.P., Parish, D., Leese, M.P., Smith, A.C., Potter, B.V., and Reed, M.J. Pharmacokinetics and efficacy of 2-methoxyoestradiol and 2-methoxyoestradiol-bis-sulphamate in vivo in rodents. Br. J. Cancer 90, 932-937, 2004.
  20. Wang, J-W., Yang, M., Yagi, S., and Hoffman, R.M. Oral 5-FU is a more effective antimetastatic agent than UFT. Anticancer Research 24, 1353-1360, 2004.
  21. Katz MH, Bouvet M, Takimoto S, Spivack D, Moossa AR, Hoffman RM. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer. Cancer Res. 64, 1828-1833, 2004.
  22. Lukashev M, LePage D, Wilson C, Bailly V, Garber E, Lukashin A, Ngam-ek A, Zeng W, Allaire N, Perrin S, Xu X, Szeliga K, Wortham K, Kelly R, Bottiglio C, Ding J, Griffith L, Heaney G, Silverio E, Yang W, Jarpe M, Fawell S, Reff M, Carmillo A, Miatkowski K, Amatucci J, Crowell T, Prentice H, Meier W, Violette SM, Mackay F, Yang D, Hoffman RM, Browning JL. Targeting the lymphotoxin-β receptor with agonist antibodies as a potential cancer therapy. Cancer Res. 66, 9617-9624, 2006.
  23. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M. A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007.
  24. Duan, J.X., Jiao, H., Kaizerman, J., Stanton, T., Evans, J.W., Lan, L., Lorente, G., Banica, M., Jung, D., Wang, J., Ma, H., Li, X., Yang, Z., Hoffman, R.M., Ammons, W.S., Hart, C.P., and Matteucci, M. Potent and highly selective hypoxia-activated achiral phosphoramidate mustards as anticancer drugs. Journal of Medicinal Chemistry 51, 2412-2420, 2008.
  25. Matsuo, Y., Raimondo, M., Woodward, T.A., Wallace, M.B., Gill, K.R., Tong, Z., Burdick, M.D., Yang, Z., Strieter, R.M., Hoffman, R.M., Guha, S. CXC-chemokine/CXCR2 biological axis promotes angiogenesis in vitro and in vivo in pancreatic cancer. Int. J. Cancer 125, 1027-1037, 2009.

Reviews

  1. Hoffman, R.M. Orthotopic is orthodox: Why are orthotopic-transplant metastatic models different from all other models? J. Cellular Biochem. 56, 1-3, 1994.
  2. Hoffman, R.M. Fertile Seed and Rich Soil: The development of patient-like models of human cancer by surgical orthotopic implantation of intact tissue. Update Series: Comprehensive Textbook of Oncology, 3: 1-10. Schimpff, S.C. et al., eds. Baltimore: Williams & Wilkins, 1996.
  3. Hoffman, R.M. Fertile seed and rich soil: The development of clinically relevant models of human cancer by surgical orthotopic implantation of intact tissue. In: Anticancer Drug Development Guide: Preclinical Screening, Clinical Trials, and Approval, pp.127-144. Teicher, B., ed. Totowa, NJ: Humana Press, 1997.
  4. Hoffman, R.M. Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Investigational New Drugs 17, 343-359, 1999.
  5. Hoffman, R.M. Clinically Accurate Orthotopic Mouse Models of Cancer. In: Methods in Molecular Medicine, Vol. 58: Metastasis Research Protocols, Vol. 2: Cell Behavior In Vitro and In Vivo, pp. 251-275. Brooks, S.A., and Schumacher, U., eds. Totowa, NJ: Humana Press, 2001.
  6. Hoffman, R.M. Green Fluorescent Protein for Metastasis Research. In: Methods in Molecular Medicine, Vol. 58: Metastasis Research Protocols, Vol. 2: Cell Behavior In Vitro and In Vivo, pp. 285-298. Brooks, S.A., and Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2001.
  7. Hoffman, R.M. GFP-Expressing Metastatic-Cancer Mouse Models. In: Tumor Models in Cancer Research, pp. 99-112. Teicher, B., ed. Totowa, NJ: Humana Press Inc., 2002.
  8. Hoffman, R.M. Green fluorescent protein imaging of tumor cells in mice. Lab Animal 31, 34-41, 2002.
  9. Hoffman, R.M. Watching real-time metastasis in vivo. Trends in Molecular Medicine 8(7), 354-355, 2002.
  10. Hoffman, R.M. Whole-body fluorescence imaging with Green Fluorescence Protein. In: Methods in Molecular Biology, Vol. 183: Green Fluorescent Protein: Applications and Protocols, pp. 135-148. Hicks, B.W., ed. Totowa, NJ: Humana Press, 2002.
  11. Hoffman, R.M. Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models. Lancet Oncology 3, 546-556, 2002.
  12. Hoffman, R.M. Patient-like orthotopic metastatic models of human cancer. In: Anticancer Drug Development Guide Preclinical Screening, Clinical Trials, and Approval, 2nd Ed., pp. 183-212. Teicher, B., Andrews, P.A., eds. Totowa, NJ: Humana Press, 2004.
  13. Glinsky, G.V., Glinskii, A.B., Stephenson, A.J., Hoffman, R.M., and Gerald, W.L. Gene expression profiling predicts clinical outcome of prostate cancer. J. Clin. Investig. 113, 913-923, 2004.
  14. Hoffman, R.M. Orthotopic metastatic (MetaMouse®) models for discovery and development of novel chemotherapy. In: Methods in Molecular Medicine, Vol. III: Chemosensitivity: Vol. II, pp. 297-322, In Vivo Models, Imaging, and Molecular Regulators. Blumenthal, R., ed. ISBN #1-58829-586-9. Totowa, NJ: Humana Press, 2005. DOI #10.1226/1588295869.
  15. Hoffman, R.M. Advantages of multi-color fluorescent proteins for whole-body and in vivo cellular imaging. Journal of Biomedical Optics 10(4), 41202, AN #041202, 2005.
  16. Hoffman, R.M. In vivo cell biology of cancer cells visualized with fluorescent proteins. In: “In vivo cellular and molecular imaging” for Current Topics in Developmental Biology, pp. 121-144. Ahrens, Eric T., ed. London: Elsevier Life Sciences, 2005. DOI: 10.1016/S0070-2153(05)70006-5.
  17. Hoffman, R.M. AngioMouse: imageable models of angiogenesis. In: Angiogenesis Assays: A critical appraisal of current techniques, pp. 293-310. Staton, C.A., Lewis, C., and Bicknell, R., eds. Wiley and Sons, 2006.
  18. Hoffman, R.M. Orthotopic metastatic mouse models of prostate cancer. In: Metastasis of Prostate Cancer, pp. 143-169. Ablin, R.J. and Mason, M.D., eds. Kluwer Academic Publishers, 2007.
  19. Mack, G., Marshall, A. Lost in migration. Nature Biotechnology 28, 214-229, 2010.
  20. Hoffman, R. Orthotopic mouse models expressing fluorescent proteins for cancer drug discovery. Expert Opin Drug Discov. 5, 851-866, 2010.
  21. Hoffman, R.M. Imaging the steps of metastasis at the macro and cellular level with fluorescent proteins in real time (Chapter 6). In: Tumor Models in Cancer Research, vol. 2. Teicher, B.A., ed. Humana Press (c/o Springer Science+Business Media LLC), 125-166, 2011.
  22. Hoffman, R.M. Imageable clinically-relevant mouse models of cancer. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 141-170. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.
  23. Hoffman, R.M. Imaging metastatic cell trafficking at the cellular level in vivo with fluorescent proteins. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 171-179. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.
  24. Hoffman, R.M. The in vivo imaging revolution made by fluorescent proteins. In: The Fluorescent Protein Revolution, Day, R.N., Davidson, M.W., eds. Taylor and Francis Books, 2014, pp 309-324.
  25. Hoffman, R.M. Patient-derived orthotopic xenografts: better mimic of metastasis than subcutaneous xenografts. Nature Reviews Cancer 15, 451-452, 2015.
  26. Hoffman, R.M. Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties. Cancer Metastasis Reviews (Special Issue: Timar, Joszef, ed.), Cancer Metastasis Rev. 35, 5-19, 2016. DOI: 10.1007/s10555-016-9610-8.